FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6955822
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG754031.0840271645824173No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC558790.8033414311062014No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA250820.36059002084872216No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG239100.34374082603033834No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC215630.30999930705529843No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT204550.294070204786724No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA201660.2899154118664911No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT186770.2685088836373329No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC168040.2415818001093185No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA161450.2321077221355003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150120.21581920871465657No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA137220.1972735932575618No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA111300.16000984499028295No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC86850.12485943429834748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT433300.069.5396048
AGTAGGT433750.069.491667
TAGGTTG434000.069.4113169
AGATGCT150450.069.40736
CCTTCAT329750.069.405622
AGGTTGT454750.069.3912710
TAGTAGG435050.069.385516
TGAGGTA610600.069.381571
TGCTGGC150800.069.3572549
AAGATGC150600.069.338685
TATAGTT404300.069.3015216
GTTGTAT337550.069.3005812
GGTAGTA607800.069.217794
CAAGATG151050.069.178444
GCTGGCA150950.069.14925410
AGGTAGT607350.069.148073
GGAGTCT329400.069.06106615
CGGAGTC330100.069.0312214
CCACCGG331150.069.0116310
GTAGTAG604150.068.987225
Produced by FastQC (version 0.11.5)