FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7396590
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC885441.197092173555652No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG844491.1417288236876721No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT294830.39860259930589637No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC285510.38600219831030247No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA273830.37021113783513754No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC268730.3633160686208104No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA265610.3590979086308691No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG248540.33601970637820944No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT218740.2957308705768469No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA204910.2770330652368186No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA199400.2695836865366338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155040.20961010411554515No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA147960.20003812567683218No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG94570.12785621482331722No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC86720.11724321613067644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT503350.069.4306958
AGTAGGT504900.069.3690957
TAGGTTG503950.069.352639
AGGTTGT527900.069.2951310
GTTGTGT146300.069.2913412
TGAGGTA704600.069.2873461
GGTAGTA697450.069.259954
CCTTCAT367500.069.241182
GTTGTAT387200.069.2348812
CGGAGTC366150.069.1630814
AGATGCT164000.069.159856
TAGTAGG506750.069.115016
AGGTAGT698100.069.10573
AGCTTAT342000.069.1032262
CTTCATT367950.069.0894553
AAGATGC164150.069.0544745
TATAGTT460850.069.04249616
GTAGTAG694200.069.004465
TGTATAG504750.069.00053414
TGCTGGC164600.068.985339
Produced by FastQC (version 0.11.5)