FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7889157
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG873181.1068102713635943No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC845151.0712804929601476No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA396450.5025251747430048No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA301760.38249967645465793No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA288960.36627487575668727No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA281780.3571737766151694No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT280930.35609634844381977No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC267820.33947860335394514No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG253970.3219228619737191No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC248480.31496394354935514No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT226340.28690010859208404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173680.22015026447058916No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA148390.18809360746655188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA103670.13140821002801695No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC91850.11642562063348466No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG90100.11420738616305899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT509400.069.474358
AGTAGGT510400.069.420947
TAGTAGG509900.069.399986
TGAGGTA716700.069.3867951
GTTGTAT396550.069.358812
TAGGTTG510300.069.3454069
AGGTTGT533600.069.32078610
GGTAGTA710800.069.302264
CCTTCAT345200.069.302172
AGATGCT155750.069.293686
TATAGTT476600.069.214416
GGAGTCT344950.069.1742215
GCTGGCA155550.069.1282910
TGCTGGC155950.069.108049
GTTGTGT142350.069.0512112
CTTCATT346300.069.03153
AGGTAGT712700.069.014383
AAGATGC156300.069.006385
GTAGTAG708600.068.993925
AGCTTAT359050.068.938882
Produced by FastQC (version 0.11.5)