FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522541
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG728221.318632129666398No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC478130.8657789955746821No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG260060.4709064178971238No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA258920.46884215074184143No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT212840.3854022994125349No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC201030.36401721598807507No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA186310.3373628190356577No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA168590.30527614009565524No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA146340.2649867153543994No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC138240.2503195539879197No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT126070.22828259672494963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119540.21645832959863945No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC109290.19789803280772386No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA106000.19194063022800556No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA78340.14185499030247128No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT72180.13070070462129663No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC68120.12334901633143149No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG66740.12085016661714236No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG64240.11632326496082149No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG56960.10314092733761505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT368550.069.52268
CCTTCAT260050.069.425192
AGTAGGT369600.069.371187
TAGGTTG369100.069.362129
AAGATGC121400.069.3549655
AGGTTGT386950.069.34576410
GTTGTAT286550.069.3296612
TAGTAGG370200.069.2807856
GGAGTCT259700.069.273715
GGTAGTA516000.069.2708744
TGAGGTA520550.069.2157441
CAAGATG121650.069.213064
AGGTAGT516150.069.1829453
AGATGCT121750.069.156216
CGGAGTC259700.069.15304614
CCACCGG260750.069.14236510
GTAGTAG513500.069.0350345
TATAGTT343350.069.0304416
GAGGTAG542000.069.028352
CTTCATT261600.068.973093
Produced by FastQC (version 0.11.5)