FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8290416
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG729840.8803418308562562No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC565530.6821491225530781No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA298910.36054885544947324No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA290730.3506820405634651No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA264900.31952558231094796No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC226030.272640118421078No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG213470.25749009458632716No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT211690.25534303706834494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161030.19423633265206475No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC154630.1865165752840388No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT151460.18269288296268849No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA116640.14069257803227245No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA101260.12214103610723515No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC85120.10267277299474478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT441250.069.497218
AGTAGGT442350.069.435147
TAGGTTG441900.069.387069
GTTGTAT335900.069.3646712
CCTTCAT368450.069.34852
AGGTTGT456600.069.3068110
TGAGGTA614850.069.279621
TAGTAGG443450.069.2604456
AGATGCT150150.069.2252356
GGTAGTA614500.069.202354
CTTCATT369250.069.112953
TATAGTT404900.069.09090416
GGAGTCT368150.069.0201915
AAGATGC150600.068.995155
TGCTGGC151250.068.9940269
TGTATAG439900.068.9074414
GTAGTAG611200.068.883565
GTTGTGT126050.068.8792612
CCACCGG370000.068.82558410
AGCTTAT279750.068.7809142
Produced by FastQC (version 0.11.5)