FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5469085
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG563391.030135753969814No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC554271.013460204037787No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA248670.45468300456109206No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA221160.4043820858516552No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA194640.35589134197036615No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG187400.34265329575239734No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT186390.3408065517358022No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC182380.333474429452093No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT162210.2965944029028622No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC162090.29637498777217763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113490.20751185984492834No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA94910.17353908377726804No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA93800.1715094938184358No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC75420.13790240963524977No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG58640.10722086052785795No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA58450.10687345323760739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT361900.069.550368
AGTAGGT362850.069.4847
CCTTCAT215700.069.422762
GTTGTAT278550.069.4082412
TGAGGTA513550.069.368281
TAGGTTG362650.069.3679359
TAGTAGG363600.069.364366
AGGTTGT379450.069.3418510
TATAGTT334150.069.2321216
GGTAGTA508400.069.192764
AGATGCT110100.069.131776
AAGATGC109900.069.1002665
GGTTGTG105850.069.0935311
GTTGTGT105600.069.09141512
TGTATAG363950.069.0823314
CTTCATT216750.069.0535353
TGCTGGC110600.068.909759
GTAGTAG505450.068.8979345
TTGTATA364650.068.8729513
GGAGTCT216700.068.8557415
Produced by FastQC (version 0.11.5)