FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8106223
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG848281.0464552973684538No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC829391.023152212812305No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA452090.5577073317622769No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA361750.44626208778120213No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA332520.4102033709163935No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC315200.3888370699893156No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG284530.3510019401144035No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT277920.34284771094997013No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT270680.3339163010936166No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC244760.3019408669117541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154780.19093972618320518No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA152930.18865752891328058No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA146970.18130515284368565No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC119600.14754096945026063No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA98900.12200503243002321No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG95230.11747764649455116No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA94320.11635505216177744No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT82060.10123086917297981No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT492900.069.536078
AGTAGGT493500.069.487427
TAGGTTG493450.069.38689
AGGTTGT513300.069.3821410
TAGTAGG494500.069.330376
AAGATGC158700.069.327415
TGAGGTA684200.069.318111
AGATGCT158850.069.305586
GTTGTAT373150.069.2791612
GTTGTGT146600.069.2425312
CCTTCAT344250.069.225672
GGTAGTA681000.069.192014
GGTTGTG147400.069.0570611
TATAGTT447700.069.0523816
GTAGTAG676750.069.016795
TGCTGGC159400.069.016589
CTTCATT345050.068.994183
TGTATAG488600.068.92255414
AGCTTAT304150.068.918062
CGGAGTC345400.068.9086114
Produced by FastQC (version 0.11.5)