FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7416680
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG677160.9130230777113211No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC516850.6968751516851206No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA273260.3684397870745401No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG244020.3290151388491886No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT235210.3171365085186364No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA221900.29919047336544113No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC212310.2862601595322975No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT157700.21262883122906748No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC150780.2032985109240253No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA141370.19061089328378736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130700.17622440229320935No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA109750.1479772620633491No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC100810.13592335114903165No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA79380.1070290210714228No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA76930.10372565622353937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT410300.069.490658
AGTAGGT411500.069.4415367
TAGGTTG410750.069.397489
TGAGGTA570900.069.395341
GTTGTAT306350.069.2775812
AGGTTGT427250.069.20676410
TATAGTT370000.069.18188516
TAGTAGG413250.069.1786
CCTTCAT285750.069.169962
GGTAGTA570550.069.0827264
CTTCATT285900.069.060233
AGATGCT133850.069.0497366
GTAGTAG567150.068.9481665
TGCTGGC134250.068.942699
AAGATGC134450.068.8977665
GTTGTGT124950.068.8935612
AGCTTAT224050.068.819042
GGAGTCT286950.068.7780315
TGTATAG403500.068.7451614
CAAGATG134750.068.717944
Produced by FastQC (version 0.11.5)