FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8553666
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC926821.0835354104310362No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG856291.0010795371247838No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC334900.39152803020365773No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA334410.3909551764120787No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT284030.33205645392279753No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG279340.32657342477482754No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC265400.3102763189490915No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT264860.30964501068898415No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA191020.2233194515661472No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165810.19384670853409522No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA161640.18897160585882125No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA148040.17307199041907878No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC124840.14594911702187108No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG103780.12132809487768169No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA100800.11784420855338518No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG96130.11238456119282657No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT93080.1088188386125902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT475500.069.406768
TGCTGGC157750.069.366219
TAGGTTG475800.069.3548369
AGTAGGT476450.069.349567
TGAGGTA658450.069.242281
CCTTCAT347100.069.22762
TAGTAGG476950.069.216096
GTTGTAT359500.069.2090212
AGGTTGT494850.069.1811810
AAGATGC158750.069.090035
GGTAGTA657850.069.024864
CTTCATT348150.068.9480443
TGTATAG467800.068.92551414
AGATGCT159150.068.894396
TATAGTT432250.068.8875316
GGAGTCT347800.068.8809815
CAAGATG159650.068.853194
AGCTTAT272150.068.759382
GTAGTAG655750.068.7236945
GCTGGCA159550.068.627510
Produced by FastQC (version 0.11.5)