FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920863
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC926141.169241280905881No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG633450.7997234644760299No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA436130.5506091949829204No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA417960.5276697753767488No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT306010.38633416586046243No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC300850.37981972418914456No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC254160.3208741269732856No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA235370.297151964375599No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA210300.2655013727670836No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG204280.25790119081721274No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT200400.2530027346767644No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA147930.18675995279807264No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA143370.18100300434434985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142190.17951326768307949No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG92590.1168938283618843No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC82370.10399119388884771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT384100.069.402098
AGTAGGT384750.069.367577
CCTTCAT283850.069.2784352
TAGGTTG384700.069.247499
GTTGTAT295100.069.23810612
AGGTTGT399050.069.1867310
TAGTAGG386100.069.176026
TGAGGTA544500.069.146251
CTTCATT283900.069.117443
GGTAGTA542600.069.1146244
TGCTGGC135900.069.031579
AGATGCT135850.068.961356
AAGATGC136150.068.83515
TATAGTT362450.068.82748416
AGCTTAT276650.068.804422
GTTGTGT109200.068.799512
GTAGTAG541250.068.7115555
TGTATAG393500.068.65124514
GGAGTCT285100.068.5736615
CAAGATG136600.068.505864
Produced by FastQC (version 0.11.5)