FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777309
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC763161.1260516526544682No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG666870.9839746129326551No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA268980.39688318770768755No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC261970.38653984937089336No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA257040.37926557576170716No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA256240.37808516625108873No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC215250.317603933950776No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT214030.31580380944708286No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT213340.3147857062441745No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG212880.3141069707755689No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130900.19314450617494347No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA129780.19149193286007765No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA98530.14538218635154454No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC88270.13024343437786295No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG82710.12203958827906475No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA82290.12141987328599006No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA69870.10309401563363867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT364800.069.3637858
AGTAGGT365600.069.279017
TAGGTTG365200.069.241439
TGAGGTA511150.069.185171
CCTTCAT258600.069.168492
TAGTAGG366250.069.142566
AGGTTGT379500.069.1415210
GTTGTAT281350.069.12073512
GGTAGTA515000.069.0813754
AGCTTAT245100.069.00882
TATAGTT341800.068.9849916
AGATGCT119950.068.9134756
TGCTGGC120250.068.91219
TGTATAG370500.068.8852914
AAGATGC120150.068.8565
GCTTATC245850.068.7823
GGAGTCT259350.068.7754815
CTTCATT259850.068.766393
GTTGTGT103950.068.6784712
GTAGTAG512450.068.660275
Produced by FastQC (version 0.11.5)