FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8341621
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1112281.3334098971890476No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG904261.0840339065992088No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC379340.4547557363251099No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT330760.3965176552614893No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA326220.3910750680233494No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA320950.384757351119165No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC312260.37433971166995No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA306540.3674825312729984No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT264990.3176720687741627No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG248030.2973402891356488No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186910.22406915874024963No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA184260.22089231817173186No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG124950.1497910298250184No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA115790.1388099507278022No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC102510.12288978365236206No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA90290.10824035280432903No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA85540.10254601593623111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT486300.069.527028
CCTTCAT391250.069.4371952
AGTAGGT487550.069.428147
TAGGTTG487050.069.384049
AGGTTGT505750.069.32332610
TAGTAGG488800.069.2316066
AAGATGC171400.069.194235
GTTGTAT376150.069.1786112
GGAGTCT391400.069.1556715
TATAGTT457250.069.1380816
AGATGCT171500.069.1334846
GGTAGTA688700.069.121184
TGAGGTA693200.069.0853651
AGCTTAT376350.069.071192
GTTGTGT136650.069.0148612
CAAGATG172050.068.993424
TGCTGGC171800.068.9691249
GCTTATC376700.068.950033
TGTATAG498900.068.9494714
CTTCATT393550.068.887883
Produced by FastQC (version 0.11.5)