FastQCFastQC Report
Wed 6 Feb 2019
HLYJNBGX9_n01_HBL01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYJNBGX9_n01_HBL01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6897219
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG706851.0248333422499705No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC296750.43024587156069716No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA255830.3709176118664639No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG231570.33574401508782015No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA230860.3347146146874559No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA215870.31298121750230057No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT211240.3062683670041505No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC132760.1924833762709289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132220.19170045202276453No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC81990.11887399834628999No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC77030.11168269414092839No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG76770.11130573061403444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA76290.11060979794899944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT423650.069.507238
CCTTCAT329600.069.359352
TAGGTTG424450.069.359239
AGGTTGT441050.069.3352110
AGTAGGT425500.069.3283847
GGTAGTA593500.069.3232044
TGAGGTA598100.069.302991
TAGTAGG425750.069.263836
GTTGTAT321450.069.213912
AGATGCT176100.069.160746
TGCTGGC176450.069.152879
GCTGGCA176100.069.15121510
GGAGTCT329200.069.1364215
GGTTGTG125450.069.1225911
TATAGTT392750.069.069116
CTTCATT331000.069.022693
CAAGATG176750.069.005414
GTAGTAG591050.069.001165
AGCTTAT301450.068.986392
AAGATGC176750.068.986115
Produced by FastQC (version 0.11.5)