FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9251b-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9251b-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5693749
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT419690.7371066058584599TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT69650.038.8804350-51
TGCCGTC74250.038.48721350-51
TATGCCG77100.037.00372348-49
GTATGCC78300.036.769746-47
CGTATGC79300.036.725446-47
ATGCCGT78550.035.9879948-49
CCGTCTT77950.034.73912452-53
TCGTATG84750.033.74528544-45
CTCGTAT77600.033.1507344-45
ATCTCGT77450.032.75301742-43
AGGGGGG49200.032.72749374-75
TCTCGTA79400.032.4272642-43
AAGGGGG49000.031.16470574-75
CTGCTTG81050.030.50524158-59
AATCTCG83500.030.4063440-41
CACAGTC111400.029.29114530-31
AGTCAAC110550.028.89363334-35
GCTTGAA92950.027.85183760-61
CGTCTTC97700.027.57075552-53
ACGGGGG9150.027.5126874-75
Produced by FastQC (version 0.11.2)