FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9251a-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9251a-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7793657
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT104730.1343785080611066TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT26900.021.54691550-51
TGCCGTC32950.020.97901550-51
AGGGGGG27700.020.06235574-75
TATGCCG38050.019.22878848-49
CCGTATC45300.018.24545538-39
GTATGCC41150.017.95310246-47
CGTATGC41650.017.68054446-47
ATGCCGT40950.017.46097448-49
TATCTCG41550.017.37761540-41
AAGGGGG28500.017.08263274-75
GTTCCGT53300.016.8425434-35
CGTATCT46750.016.40947238-39
ATCTCGT45900.015.99026642-43
CTCGTAT43800.015.94419644-45
CAGTCAC77150.015.20603326-27
AGTTCCG61950.015.0658734-35
TCGTATG48900.015.00994244-45
TCTCGTA48150.014.84841642-43
CCGTCTT40750.014.80597152-53
GTCACAG72300.014.51825628-29
Produced by FastQC (version 0.11.2)