FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9120b-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9120b-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24562354
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3267991.3304872977565587TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC365830.14893930769013425TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1313600.061.68449
AGAGCAC1359300.059.7921338
CGGAAGA1501150.054.4133384
GAAGAGC1508650.053.9256256
TCGGAAG1516250.053.680293
AAGAGCA1547800.052.9281657
ATCGGAA1575900.051.5827182
GGAAGAG1578150.051.5567175
GATCGGA1489150.051.1616441
TGCCGTC502650.042.19746448-49
GCCGTCT451050.042.01637648-49
CCGATGT888950.041.44365732-33
GTATGCC589950.041.14153344-45
ATGCCGT543450.041.0223846-47
TATGCCG579850.040.79049746-47
TATCTCG613050.040.6116438-39
CGTATGC582650.040.52771444-45
CGATGTA899900.040.52281634-35
ACCGATG933500.040.0840232-33
CACCGAT951150.039.92622830-31
Produced by FastQC (version 0.11.2)