FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9120a-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9120a-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14181365
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1096130.7729368787842356TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA418900.048.4607359
AGAGCAC435150.046.847688
TCGGAAG492250.041.236023
CGGAAGA502400.040.6866574
GCCGTCT200350.040.287748-49
GAAGAGC506350.040.181736
GATCGGA476350.039.5830151
TGCCGTC208800.039.55602348-49
ATCGGAA523100.038.6590542
AAGAGCA534350.038.4169857
GGAAGAG534150.038.099355
TATGCCG225600.037.67355346-47
CGTATGC231600.037.3526544-45
GTATGCC231050.037.06118844-45
ATGCCGT226750.036.91679446-47
CCGTCTT221500.036.1185850-51
ACTGACC250200.036.0498532-33
TCGTATG243600.035.1302342-43
CTGACCA260350.034.3251532-33
CTCGTAT204000.034.14883442-43
Produced by FastQC (version 0.11.2)