FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9048spikeb-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9048spikeb-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15205870
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT902870.5937641187252028TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA357200.049.1850289
AGAGCAC373950.047.172298
CGGAAGA437000.041.0461734
TCGGAAG435700.040.8850983
GATCGGA424700.039.9563831
GAAGAGC453600.039.418476
CGTCCGC200500.039.1704432-33
ACGTCCG201150.039.0792832-33
ATCGGAA461050.038.1634032
GCCGTCT171500.037.96394750-51
CCGCACA193500.037.5690536-37
TGCCGTC180550.037.46872750-51
GTCCGCA205150.037.09060334-35
CACGTCC217400.037.00974330-31
AAGAGCA484600.036.7153177
GGAAGAG489000.036.535715
GTATGCC196650.036.4069546-47
AGTCACG226350.036.38552528-29
TATGCCG193050.036.15107748-49
TCCGCAC214600.035.888934-35
Produced by FastQC (version 0.11.2)