FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah9048spikea-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah9048spikea-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11803167
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT293000.2482384600675395TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT74100.029.26839350-51
TGCCGTC80500.027.94476550-51
GTATGCC87650.025.58442146-47
ATGCCGT90350.025.1880648-49
TATGCCG90650.024.9998848-49
AGGGGGG52850.024.80489374-75
CGTATGC93250.024.43010346-47
GATCTCG75200.023.78263340-41
CGATCTC75550.023.60958140-41
CCGTCTT93800.023.29779252-53
CTCGTAT91500.021.88489744-45
AAGGGGG54650.021.4674374-75
TCGTATG103450.021.37751644-45
TCTCGTA94000.020.8213542-43
CTGCTTG101750.020.30746558-59
ATCTCGT91400.020.19223242-43
ACGATCT101300.018.21704138-39
CACGTGA133450.018.13364430-31
CGTCTTC125400.017.42689752-53
GCTTGAA123650.017.42129160-61
Produced by FastQC (version 0.11.2)