FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah8584d-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah8584d-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7886347
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC598260.7586021766478194TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC238360.3022438652521884TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA306150.064.631289
AGAGCAC317650.062.5455938
TCGGAAG345900.057.554353
CGGAAGA348650.057.2639244
GATCGGA329900.056.1898571
GAAGAGC360900.055.0177656
ATCGGAA361500.054.8742262
GGAAGAG371550.053.4276285
AAGAGCA377950.052.94417
GCCGTCT125650.041.2148748-49
TGCCGTC141200.040.98288348-49
CACACAG230750.040.9510930-31
TATGCCG155250.040.45637546-47
GTCACAC233900.040.33841728-29
GTATGCC159400.040.19128444-45
CACAGTG228650.040.1439232-33
ATGCCGT150000.040.09863746-47
CGTATGC157400.040.06812744-45
GATCTCG116400.039.52186638-39
CAGTCAC246000.039.1260926-27
Produced by FastQC (version 0.11.2)