FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah8584c-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah8584c-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219264
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC151630.29051988939436674TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT30950.029.24146348-49
TGCCGTC33650.027.60110748-49
TATGCCG39050.024.94030446-47
GTATGCC40500.024.3987144-45
CGTATGC40800.024.33574744-45
ATGCCGT39400.024.23643546-47
TATCTCG41950.023.38213738-39
CCGTCTT39000.023.08357850-51
TCGTATG44550.022.71274642-43
CTCGTAT42650.022.6664342-43
AAGGGGG23750.022.4987372-73
ATCTCGT45700.021.25620540-41
TCTCGTA45400.021.18740540-41
GTCACGC51650.021.0126528-29
CACGCCA52950.020.54160930-31
AGGGGGG24050.020.53938172-73
TCACGCC52850.020.31086330-31
CTGCTTG42950.019.57613856-57
AGTCACG58450.019.3806728-29
CGCCAAT58450.018.93382632-33
Produced by FastQC (version 0.11.2)