FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah8583d-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah8583d-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721226
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC337470.5898560902855436TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA159900.068.912659
AGAGCAC162850.067.8976448
GATCGGA158250.065.098161
TCGGAAG172800.063.2416273
CGGAAGA176300.062.6059844
ATCGGAA179450.061.0569042
GAAGAGC188200.058.6478846
GGAAGAG195450.056.4724045
AAGAGCA203250.054.659167
CAGTCAC135700.039.18320526-27
TGCCGTC66650.038.6720948-49
ACGTCTG141050.038.65667314-15
GAACTCC141050.038.55565320-21
GCCGTCT58300.038.50625648-49
AGTCACC136350.038.3519928-29
CGTCTGA142450.038.2767616-17
CACGTCT142850.038.21944814-15
TCCAGTC138650.038.09259824-25
CATCTCG75700.038.05597738-39
ACACGTC145350.038.01955412-13
Produced by FastQC (version 0.11.2)