FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah8583c-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah8583c-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13264122
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC479920.36181814371128374TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT103250.034.2569348-49
TGCCGTC113700.033.30210548-49
TATGCCG124950.031.95762346-47
CGTATGC128950.031.07606744-45
GTATGCC130700.030.76902444-45
ATGCCGT126800.030.32987246-47
TCGTATG138300.028.2351942-43
CCGTCTT128800.028.14346550-51
AAGGGGG79600.027.71660472-73
GAGCACA388900.026.4658019
CTCGTAT118800.026.29180342-43
ATCTCGT121850.025.55413440-41
AGGGGGG84500.025.18191172-73
AGAGCAC416100.024.8385898
CTGCTTG131500.024.45570856-57
GTCACCT197350.024.22380628-29
TCTCGTA128550.024.11140340-41
CCTTGTA192700.023.97030832-33
AATCTCG133500.023.8745338-39
AGTCACC205500.023.79470328-29
Produced by FastQC (version 0.11.2)