FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah119spikeb-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah119spikeb-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32263747
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3707911.149249651629118TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT856810.2655643189862604TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT617660.1914408763495449TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT383810.11896014433785387TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT344420.10675139499451194TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2195650.060.8402949
AGAGCAC2276700.058.851818
CGGAAGA2484400.054.123814
GAAGAGC2484050.053.7737466
AAGAGCA2530850.053.317297
TCGGAAG2515600.053.1541063
GGAAGAG2579300.051.8192755
ATCGGAA2593450.051.4872362
GATCGGA2457200.051.0550041
TGCCGTC494600.040.32284550-51
ATGTCAG1559600.040.0284534-35
ACATGTC1577000.039.783732-33
GCCGTCT406700.039.6928350-51
TGTCAGA1538100.039.63685634-35
GTATGCC602600.039.51447746-47
TCACATG1601750.039.4947630-31
CACATGT1589100.039.4714130-31
ATGCCGT549000.039.30063648-49
TATGCCG577450.039.28132248-49
AAGGGGG705050.038.95197374-75
Produced by FastQC (version 0.11.2)