FastQCFastQC Report
Fri 7 Jul 2017
HLYHHAFXX_n01_ah119spikea-062817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLYHHAFXX_n01_ah119spikea-062817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3619266
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT233140.6441637613814514TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA55950.038.75488336-37
GCCGTCT53450.038.70536450-51
TATGCCG57300.038.59239648-49
TGCCGTC56400.038.49176450-51
CCCGATC44850.038.33847438-39
GTATGCC58150.038.314246-47
CGTCCCG60150.037.98246434-35
ATGCCGT58200.037.62830448-49
GAGCACA143150.037.260889
CGTATGC61000.036.99137546-47
CCGATCT44650.036.54213738-39
GATCTCG45150.036.40097840-41
TCCCGAT53950.036.0975836-37
CGATCTC46200.036.03634640-41
CCGTCTT58200.035.46479852-53
AGAGCAC153200.035.3746038
CCGTCCC66200.034.9417534-35
CTCGTAT55950.034.93843544-45
TCGTATG62000.034.59391844-45
AGGGGGG38200.034.5662474-75
Produced by FastQC (version 0.11.2)