FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141684
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1946833.169863509747489No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1315792.142392868144958No Hit
AAAAAAAAAAAAAAAAAAAAATTTT659001.072995614883475No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA533110.8680192598642327No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG473110.7703261841540529No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA467920.7618757331051224No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT369070.6009263908726011No Hit
AAAAAAAAAAAAAAAAAAAAATTTA322090.5244327125915302No Hit
AAAAAAAAAAAAAAAAAAAAAATTT307280.5003188050704009No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC303050.4934314432328332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT279400.4549240892237373No Hit
AAAAAAAAAAAAAAAAAAAATTTTT275150.4480041630275996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG271950.44279386565639No Hit
TATTTTTTTTTTTTTTTTTTTTTTT221720.36100847910768447No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT193740.31545094146817065No Hit
AAAAAAAAAAAAAAAAAAAAAAATT187490.3052745794150269No Hit
AAAAAAAAAAAAAAAAAAAAAATTA176490.28736418220149396No Hit
TTTATTTTTTTTTTTTTTTTTTTTT171220.2787834737182831No Hit
TTATTTTTTTTTTTTTTTTTTTTTT155800.25367635326076693No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA152650.2485474667859825No Hit
TATTTTTTTTTTTTTTTTTTTTTTA150250.2446397437575753No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC144360.23504954015869262No Hit
AAAAAAAAAAAAAAAAAAAATTTTA136870.2228541878742052No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT135320.22033045008502555No Hit
AAAAAAAAAAAAAAAAAAAAAATAA122160.1989031021459261No Hit
AAAAAAAAAAAAAAAAAAAAATTAA119370.19436037412540275No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG116490.1896711064913141No Hit
TTTTTATTTTTTTTTTTTTTTTTTT113450.184721323988665No Hit
TTTATTTTTTTTTTTTTTTTTTTTA105720.1721351994013368No Hit
TTTTATTTTTTTTTTTTTTTTTTTT104430.17003479827356796No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT104270.16977428340500747No Hit
TTATTTTTTTTTTTTTTTTTTTTTA100810.16414064937238712No Hit
AAAAAAAAAAAAAAAAAAAAAAATA97950.15948394609686856No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA90470.14730487599166614No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT89540.14579063331815834No Hit
AAAAAAAAAAAAAAAAAAATTTTTT86580.14097110824978948No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC85370.13900096455630084No Hit
TTTTTTTTTTTTTTTTTTTTTAATT82540.13439310781863736No Hit
TTTTTTTTTTTTTTTTTTTTTTACC80620.1312669293959116No Hit
TTTTTTATTTTTTTTTTTTTTTTTT79830.12998063723239425No Hit
AAAAAAAAAAAAAAAAAAAAATAAA78530.12786395392534033No Hit
TTTTTTTTTTTTTTTTTTTTTTACA72310.1177364384100517No Hit
TTTTTATTTTTTTTTTTTTTTTTTA70260.11439859165662057No Hit
TTTTTTTATTTTTTTTTTTTTTTTT68570.1116469033574505No Hit
TATTTTTTTTTTTTTTTTTTTTTAA67010.10910688338898582No Hit
TTTTATTTTTTTTTTTTTTTTTTTA66840.1088300863411403No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA65880.10726699712977743No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC65510.10666455649623133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)