FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6034209
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2154743.570873995249419No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1371112.27222822411355No Hit
AAAAAAAAAAAAAAAAAAAAATTTT617061.022602962542398No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA563680.9341406636727365No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG484950.8036678875391954No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA408560.6770730016146276No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT386820.6410450814680101No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG374900.6212910424547774No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC306990.5087493654926437No Hit
AAAAAAAAAAAAAAAAAAAAAATTT301450.4995683775619969No Hit
AAAAAAAAAAAAAAAAAAAATTTTT259830.4305949628194847No Hit
AAAAAAAAAAAAAAAAAAAAATTTA253140.41950817414511166No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT234250.3882033254068595No Hit
TATTTTTTTTTTTTTTTTTTTTTTT228570.3787903269508895No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT204100.33823820156046963No Hit
TTTATTTTTTTTTTTTTTTTTTTTT186640.3093031746165902No Hit
TTATTTTTTTTTTTTTTTTTTTTTT170530.28260539202404156No Hit
AAAAAAAAAAAAAAAAAAAAAAATT167410.2774348717454102No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA161840.2682041672736228No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC146410.24263329294692973No Hit
AAAAAAAAAAAAAAAAAAAAAATTA142050.23540782230114998No Hit
TATTTTTTTTTTTTTTTTTTTTTTA141810.2350100899720245No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT136890.22685657722495192No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG125730.20836202392061662No Hit
TTTTTATTTTTTTTTTTTTTTTTTT116840.19362935556259323No Hit
TTTTATTTTTTTTTTTTTTTTTTTT115440.1913092503093612No Hit
AAAAAAAAAAAAAAAAAAAATTTTA110130.1825094225274597No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT109730.18184653531225053No Hit
TTTATTTTTTTTTTTTTTTTTTTTA107050.17740519097034924No Hit
AAAAAAAAAAAAAAAAAAAAAATAA102680.17016314814418923No Hit
TTATTTTTTTTTTTTTTTTTTTTTA100530.16660012936244004No Hit
AAAAAAAAAAAAAAAAAAAAATTAA95680.15856262187802908No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT94750.15702140910266782No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC89050.1475752662859374No Hit
TTTTTTTTTTTTTTTTTTTTTAATT89030.14754212192517693No Hit
AAAAAAAAAAAAAAAAAAATTTTTT85200.14119497683954932No Hit
TTTTTTTTTTTTTTTTTTTTTTACC84520.14006806857369375No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA82850.13730051445019553No Hit
TTTTTTATTTTTTTTTTTTTTTTTT82620.13691935430145027No Hit
AAAAAAAAAAAAAAAAAAAAAAATA82550.13680334903878869No Hit
TTTTTTTTTTTTTTTTTTTTTTACA76440.1266777468264689No Hit
TTTTTTTATTTTTTTTTTTTTTTTT73410.12165637617125957No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA69620.11537551980715285No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC68570.11363544086722883No Hit
TTTTTATTTTTTTTTTTTTTTTTTA66390.11002270554433895No Hit
TTTTATTTTTTTTTTTTTTTTTTTA66320.10990670028167736No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA64690.10720543487970007No Hit
TATTTTTTTTTTTTTTTTTTTTTAA63970.10601223789232359No Hit
TTTTTTTTATTTTTTTTTTTTTTTT63660.1054985003005365No Hit
AAAAAAAAAAAAAAAAAAAAATAAA63230.10478589654418664No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC61590.10206805896182915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)