FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136510
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2308524.494335648134628No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1554373.026120848591748No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA635901.238000120704525No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG574961.1193592536566659No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT402000.782632565691491No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC376880.7337277645716644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG363050.7068028680952632No Hit
AAAAAAAAAAAAAAAAAAAAATTTT319960.6229132231807201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA243330.47372632390475244No Hit
TATTTTTTTTTTTTTTTTTTTTTTT232500.4526419689633623No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT214130.41687838629731083No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA184150.35851190789076626No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC176600.34381321169432166No Hit
TTTATTTTTTTTTTTTTTTTTTTTT172990.3367850933805249No Hit
TTATTTTTTTTTTTTTTTTTTTTTT163590.3184847299041567No Hit
AAAAAAAAAAAAAAAAAAAATTTTT157190.3060249079627997No Hit
AAAAAAAAAAAAAAAAAAAAAATTT154890.30154715945262445No Hit
TATTTTTTTTTTTTTTTTTTTTTTA154630.30104097918625683No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG148370.28885371584986697No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT141790.2760434614164092No Hit
AAAAAAAAAAAAAAAAAAAAATTTA130720.25449186315221817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT122040.2375932296442526No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT117160.22809261541396783No Hit
TTTTTATTTTTTTTTTTTTTTTTTT114710.2233228398270421No Hit
TTTTATTTTTTTTTTTTTTTTTTTT114250.22242729012500706No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC107000.20831264808206348No Hit
TTTATTTTTTTTTTTTTTTTTTTTA104460.2033676562490874No Hit
TTTTTTTTTTTTTTTTTTTTTTACC102750.2000385475741311No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT102110.19879256537999537No Hit
TTATTTTTTTTTTTTTTTTTTTTTA100710.19606697933032352No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA93490.18201074270273007No Hit
TTTTTTTTTTTTTTTTTTTTTAATT89540.17432069634829875No Hit
TTTTTTTTTTTTTTTTTTTTTTACA88170.17165351571397702No Hit
TTTTTTATTTTTTTTTTTTTTTTTT87030.1694341099306728No Hit
AAAAAAAAAAAAAAAAAAAAAAATT85230.1659297850096661No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC83340.16225024384260908No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA77630.15113374645430458No Hit
AAAAAAAAAAAAAAAAAAAAAATTA75810.14759048458973115No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA74720.14546842116534378No Hit
TTTTTTTATTTTTTTTTTTTTTTTT73150.14241187109535464No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC72960.1420419701314706No Hit
TTTTTATTTTTTTTTTTTTTTTTTA69880.1360456808221925No Hit
TTTTATTTTTTTTTTTTTTTTTTTA69850.13598727540684238No Hit
TATTTTTTTTTTTTTTTTTTTTTAA69300.13491650945875702No Hit
AAAAAAAAAAAAAAAAAAAATTTTA65870.12823882363706096No Hit
TTTTTTTTATTTTTTTTTTTTTTTT63890.12438406622395362No Hit
TTTTTTTTTATTTTTTTTTTTTTTT61500.11973110146772808No Hit
AAAAAAAAAAAAAAAAAAAAAATAA57690.11231361371826397No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA56710.11040570348349367No Hit
AATTTTTTTTTTTTTTTTTTTTTTT53580.10431207181529872No Hit
TATTTTTTTTTTTTTTTTTTTTTTG52910.1030076842058129No Hit
TTTTTTATTTTTTTTTTTTTTTTTA52470.10215107144734459No Hit
AAAAAAAAAAAAAAAAAAAAATTAA52000.10123605327352618No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT52000.10123605327352618No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT51580.10041837745862464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)