FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5337827
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2256214.226832379543211No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1514542.8373718368916787No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA604561.132595717320925No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG524610.9828156663750998No Hit
AAAAAAAAAAAAAAAAAAAAATTTT438840.8221323021521679No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT417910.782921589628139No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC352280.6599689349242679No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA304880.5711687546261803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG300960.5638249422470979No Hit
TATTTTTTTTTTTTTTTTTTTTTTT219630.41145956959639196No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT211230.3957228287840726No Hit
AAAAAAAAAAAAAAAAAAAAAATTT205370.3847445786459546No Hit
AAAAAAAAAAAAAAAAAAAAATTTA195080.36546707115086347No Hit
TTTATTTTTTTTTTTTTTTTTTTTT183240.3432857602915943No Hit
AAAAAAAAAAAAAAAAAAAATTTTT180540.3382275221733488No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA179980.3371784061191942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT173150.3243829371015584No Hit
TTATTTTTTTTTTTTTTTTTTTTTT164860.3088522726570194No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC159280.29839858054597873No Hit
TATTTTTTTTTTTTTTTTTTTTTTA143000.26789927811448366No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT134700.25234987945469195No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG132660.24852809954312868No Hit
AAAAAAAAAAAAAAAAAAAAAAATT124360.23297870088333697No Hit
TTTTATTTTTTTTTTTTTTTTTTTT114770.21501258845593912No Hit
TTTTTATTTTTTTTTTTTTTTTTTT112740.2112095427596286No Hit
TTTATTTTTTTTTTTTTTTTTTTTA110880.20772497872261503No Hit
AAAAAAAAAAAAAAAAAAAAAATTA105940.19847027638775103No Hit
TTATTTTTTTTTTTTTTTTTTTTTA103480.19386165943557182No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT101590.19032089275279998No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT99790.18694873400730297No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC97750.1831269540957397No Hit
TTTTTTTTTTTTTTTTTTTTTTACC95730.17934264261468197No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA88500.1657978049869357No Hit
TTTTTTTTTTTTTTTTTTTTTAATT88200.16523577852935287No Hit
TTTTTTTTTTTTTTTTTTTTTTACA86110.1613203275415258No Hit
AAAAAAAAAAAAAAAAAAAATTTTA85590.16034614834838223No Hit
TTTTTTATTTTTTTTTTTTTTTTTT82080.15377043879466307No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA76180.14271725179553402No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC75810.14202408583118187No Hit
AAAAAAAAAAAAAAAAAAAAAATAA75360.14118104614480761No Hit
AAAAAAAAAAAAAAAAAAAAATTAA73250.13722812672647502No Hit
TTTTTTTATTTTTTTTTTTTTTTTT72350.13554204735372655No Hit
TTTTATTTTTTTTTTTTTTTTTTTA70430.1319450780251964No Hit
TTTTTATTTTTTTTTTTTTTTTTTA68610.12853545084919388No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA66210.1240392391885312No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC65800.12327113636316801No Hit
AAAAAAAAAAAAAAAAAAAAAAATA63950.11980530654140721No Hit
TATTTTTTTTTTTTTTTTTTTTTAA62300.11671416102470164No Hit
TTTTTTTTATTTTTTTTTTTTTTTT60900.11409137088931506No Hit
AAAAAAAAAAAAAAAAAAATTTTTT58720.11000731196421315No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA55150.10331919711897744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)