FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5511410
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2526104.583400617990677No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1717863.116915635018988No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA686471.2455433364601798No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG606361.1001903324194717No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT471830.8560967157224739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG453130.8221671042437416No Hit
AAAAAAAAAAAAAAAAAAAAATTTT393880.7146628539702182No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC384590.6978069132944201No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA285890.5187238837248545No Hit
TATTTTTTTTTTTTTTTTTTTTTTT253880.46064437231125976No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT231370.41980182929595145No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA205840.3734797447477143No Hit
TTTATTTTTTTTTTTTTTTTTTTTT189070.34305195948042333No Hit
AAAAAAAAAAAAAAAAAAAAAATTT183650.33321781540476936No Hit
TTATTTTTTTTTTTTTTTTTTTTTT182890.33183885793290646No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC175500.3184303109367657No Hit
AAAAAAAAAAAAAAAAAAAATTTTT172700.31334994130358657No Hit
AAAAAAAAAAAAAAAAAAAAATTTA166540.3021731281105924No Hit
TATTTTTTTTTTTTTTTTTTTTTTA165150.2996510874712642No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG153180.27793250728942326No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT149160.27063854803035886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT148130.26876969777243936No Hit
TTTTTATTTTTTTTTTTTTTTTTTT124940.22669335070335905No Hit
TTTTATTTTTTTTTTTTTTTTTTTT122840.22288307347847466No Hit
TTTATTTTTTTTTTTTTTTTTTTTA117980.21406500332945655No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT115460.2094926706595953No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT114950.20856731761926622No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC113650.20620857457529015No Hit
TTATTTTTTTTTTTTTTTTTTTTTA113340.2056461050801882No Hit
AAAAAAAAAAAAAAAAAAAAAAATT110140.19983996835655485No Hit
TTTTTTTTTTTTTTTTTTTTTTACC101910.18490731047046036No Hit
TTTTTTTTTTTTTTTTTTTTTAATT98870.17939148058300872No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA97590.17706902589355536No Hit
TTTTTTTTTTTTTTTTTTTTTTACA94400.1712810333471834No Hit
AAAAAAAAAAAAAAAAAAAAAATTA91310.16567448257342496No Hit
TTTTTTATTTTTTTTTTTTTTTTTT89570.16251739572994933No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA83690.15184861950027306No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC83270.1510865640552962No Hit
TTTTTTTATTTTTTTTTTTTTTTTT77480.14058108542097214No Hit
TTTTATTTTTTTTTTTTTTTTTTTA76340.13851264921317777No Hit
TTTTTATTTTTTTTTTTTTTTTTTA76190.13824048655425744No Hit
AAAAAAAAAAAAAAAAAAAATTTTA75810.137551007818326No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA74860.13582731097849735No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC74780.1356821575604065No Hit
TATTTTTTTTTTTTTTTTTTTTTAA72340.13125497830863608No Hit
TTTTTTTTATTTTTTTTTTTTTTTT66270.12024146271099409No Hit
AAAAAAAAAAAAAAAAAAAAAATAA65920.11960641650684671No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA63230.11472563282354242No Hit
AAAAAAAAAAAAAAAAAAAAATTAA62670.1137095588969066No Hit
TTTTTTTTTATTTTTTTTTTTTTTT60140.10911908204978399No Hit
AAAAAAAAAAAAAAAAAAAAAAATA58230.10565354419286535No Hit
AAAAAAAAAAAAAAAAAAATTTTTT57770.10481891203884305No Hit
AATTTTTTTTTTTTTTTTTTTTTTT56330.10220615051320805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)