FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELB_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5381478
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2612004.853685177194815No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1852363.4421027085867486No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA763981.4196471675625173No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG710971.321142630333154No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC471180.8755587219719193No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT459960.8547094311265417No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG408290.7586949161550043No Hit
AAAAAAAAAAAAAAAAAAAAATTTT331830.616614989413689No Hit
TATTTTTTTTTTTTTTTTTTTTTTT277860.5163265556414056No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA246870.4587401453652696No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT232300.4316657988753275No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA224600.41735746202065677No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC215280.4000387997498085No Hit
TTTATTTTTTTTTTTTTTTTTTTTT193990.36047717745942653No Hit
TATTTTTTTTTTTTTTTTTTTTTTA191180.3552555636202545No Hit
TTATTTTTTTTTTTTTTTTTTTTTT183710.3413746186456583No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG170810.3174035088501709No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT163430.30368980417647345No Hit
AAAAAAAAAAAAAAAAAAAAAATTT147470.27403252415042856No Hit
AAAAAAAAAAAAAAAAAAAATTTTT138670.25768013917366195No Hit
AAAAAAAAAAAAAAAAAAAAATTTA137740.2559519893977082No Hit
TTTTATTTTTTTTTTTTTTTTTTTT128490.23876340291644785No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC127560.23703525314049415No Hit
TTTTTATTTTTTTTTTTTTTTTTTT127520.23696092411787245No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT122970.2285059977946579No Hit
TTTATTTTTTTTTTTTTTTTTTTTA122600.22781845433540748No Hit
TTTTTTTTTTTTTTTTTTTTTTACC122290.22724240441008955No Hit
TTATTTTTTTTTTTTTTTTTTTTTA119140.22138899387863337No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA113780.21142890484733007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT111570.20732222634748296No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT111480.20715498604658425No Hit
TTTTTTTTTTTTTTTTTTTTTTACA107360.19949909671655258No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC99290.1845032164026314No Hit
TTTTTTATTTTTTTTTTTTTTTTTT95510.17747912376488392No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA94530.1756580627106531No Hit
TTTTTTTTTTTTTTTTTTTTTAATT90410.16800217338062146No Hit
TATTTTTTTTTTTTTTTTTTTTTAA86380.16051352435148858No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC83550.15525474600100567No Hit
AAAAAAAAAAAAAAAAAAAAAAATT82930.15410264615036984No Hit
TTTTATTTTTTTTTTTTTTTTTTTA82640.15356376073636274No Hit
TTTTTTTATTTTTTTTTTTTTTTTT82610.1535080139693965No Hit
TTTTTATTTTTTTTTTTTTTTTTTA81210.15090649817763818No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA79800.14828640013022445No Hit
AAAAAAAAAAAAAAAAAAAAAATTA73600.13676540162386616No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA69400.1289608542485912No Hit
TATTTTTTTTTTTTTTTTTTTTTTG68830.12790166567623246No Hit
TTTTTTTTATTTTTTTTTTTTTTTT66490.12355341785286494No Hit
AATTTTTTTTTTTTTTTTTTTTTTT64890.12058025694799832No Hit
TTTTTTTTTATTTTTTTTTTTTTTT63950.11883352491638913No Hit
TAATTTTTTTTTTTTTTTTTTTTTT59440.11045292761579625No Hit
AAAAAAAAAAAAAAAAAAAATTTTA59180.10996978896875542No Hit
TTTTTTATTTTTTTTTTTTTTTTTA57800.10740543768830793No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT54330.10095739497587837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)