FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902495
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2482494.205831601720967No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1671412.8317008315974856No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA693841.1755029017390104No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG623531.0563837834678387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG588890.9976967367189638No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT444120.7524275751186574No Hit
AAAAAAAAAAAAAAAAAAAAATTTT403050.6828468300269632No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC401190.6796956202419485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA310640.5262859180736281No Hit
TATTTTTTTTTTTTTTTTTTTTTTT294090.49824692778223445No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT227930.3861587345690255No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA201730.34177072576935685No Hit
TTTATTTTTTTTTTTTTTTTTTTTT196420.3327745300927828No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC191440.32433742002322746No Hit
TATTTTTTTTTTTTTTTTTTTTTTA190360.322507685309348No Hit
AAAAAAAAAAAAAAAAAAAAAATTT187210.3171709590605329No Hit
TTATTTTTTTTTTTTTTTTTTTTTT183280.31051275774058257No Hit
AAAAAAAAAAAAAAAAAAAATTTTT173110.29328275585155095No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT171680.29086005155446976No Hit
AAAAAAAAAAAAAAAAAAAAATTTA168060.28472705186535524No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG158630.26875075709509283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT145450.24642121679052673No Hit
TTTTTATTTTTTTTTTTTTTTTTTT129000.21855164638004773No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT126950.21507853882129507No Hit
TTTTATTTTTTTTTTTTTTTTTTTT123930.20996205841766913No Hit
TTTATTTTTTTTTTTTTTTTTTTTA118970.20155883232429675No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC115060.1949345149805294No Hit
TTATTTTTTTTTTTTTTTTTTTTTA113680.19259652062390567No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT110930.1879374738987496No Hit
TTTTTTTTTTTTTTTTTTTTTTACC109260.18510816188747303No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA108700.1841594105543503No Hit
AAAAAAAAAAAAAAAAAAAAAAATT102110.17299464040206727No Hit
TTTTTTTTTTTTTTTTTTTTTTACA98610.16706494457005047No Hit
TTTTTTTTTTTTTTTTTTTTTAATT94180.15955964384552634No Hit
TTTTTTATTTTTTTTTTTTTTTTTT93590.1585600665481292No Hit
AAAAAAAAAAAAAAAAAAAAAATTA93080.1576960251554639No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC89090.15093617190696476No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA87160.1476663682053098No Hit
TATTTTTTTTTTTTTTTTTTTTTAA86810.1470733986221081No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC79610.13487516719624498No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA79150.13409583574403708No Hit
TTTTTTTATTTTTTTTTTTTTTTTT78200.13248634687534677No Hit
TTTTTATTTTTTTTTTTTTTTTTTA78200.13248634687534677No Hit
TTTTATTTTTTTTTTTTTTTTTTTA77110.13063967017337583No Hit
AATTTTTTTTTTTTTTTTTTTTTTT75420.12777647418591628No Hit
AAAAAAAAAAAAAAAAAAAATTTTA74150.1256248416982988No Hit
AAAAAAAAAAAAAAAAAAAAAATAA69230.1172893835572923No Hit
TTTTTTTTATTTTTTTTTTTTTTTT68530.11610344439088893No Hit
AAAAAAAAAAAAAAAAAAAAATTAA67550.11444312955792423No Hit
TATTTTTTTTTTTTTTTTTTTTTTG66510.11268116279641066No Hit
TAATTTTTTTTTTTTTTTTTTTTTT64140.10866591161873072No Hit
TTTTTTTTTATTTTTTTTTTTTTTT63490.10756468239278474No Hit
AAAAAAAAAAAAAAAAAAATTTTTT61660.10446429857204453No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60460.10243126000106734No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)