FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5279043
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2776925.260271606046778No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1692023.2051642693571547No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA682261.2923933372014587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG644881.221585048653705No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG465520.8818264977193784No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT442670.838542137277533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC404250.7657637946877871No Hit
AAAAAAAAAAAAAAAAAAAAATTTT349490.6620328722459734No Hit
TATTTTTTTTTTTTTTTTTTTTTTT273970.5189766402736253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA237300.44951329246607763No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT218310.4135408633723954No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA208280.39454120756356786No Hit
TTTATTTTTTTTTTTTTTTTTTTTT196700.3726054135190791No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC187060.3543445279759987No Hit
TTATTTTTTTTTTTTTTTTTTTTTT183080.3468052826999136No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT166630.31564433174725043No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG165570.3136363920506047No Hit
AAAAAAAAAAAAAAAAAAAAAATTT162230.30730948772343775No Hit
TATTTTTTTTTTTTTTTTTTTTTTA161710.3063244607024417No Hit
AAAAAAAAAAAAAAAAAAAATTTTT147810.2799939307181245No Hit
AAAAAAAAAAAAAAAAAAAAATTTA141030.26715069379052225No Hit
TTTTTATTTTTTTTTTTTTTTTTTT131450.2490034652114029No Hit
TTTTATTTTTTTTTTTTTTTTTTTT131240.2486056658375391No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT130970.24809420949971425No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT119260.2259121586999765No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC115430.2186570558337941No Hit
TTTTTTTTTTTTTTTTTTTTTTACC112850.21376980638346763No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT112550.2132015215636622No Hit
TTTATTTTTTTTTTTTTTTTTTTTA106110.20100234076517276No Hit
TTATTTTTTTTTTTTTTTTTTTTTA103370.19581200607761673No Hit
TTTTTTTTTTTTTTTTTTTTTTACA102110.193425209834434No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA99250.1880075612189558No Hit
TTTTTTATTTTTTTTTTTTTTTTTT97090.18391591051635686No Hit
TTTTTTTTTTTTTTTTTTTTTAATT92090.17444449685293337No Hit
AAAAAAAAAAAAAAAAAAAAAAATT89610.16974667567587534No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC88080.16684842309486775No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA87920.16654533785763823No Hit
TTTTTTTATTTTTTTTTTTTTTTTT83090.15739595225877115No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA79990.1515236757874486No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC79180.149989306773974No Hit
AAAAAAAAAAAAAAAAAAAAAATTA77810.14739413943019597No Hit
TATTTTTTTTTTTTTTTTTTTTTAA73830.1398548941541109No Hit
TTTTATTTTTTTTTTTTTTTTTTTA72670.13765752618419663No Hit
TTTTTATTTTTTTTTTTTTTTTTTA71900.13619892848002943No Hit
TTTTTTTTATTTTTTTTTTTTTTTT70860.13422887443803733No Hit
AATTTTTTTTTTTTTTTTTTTTTTT63410.12011646807953638No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA62970.11928298367715512No Hit
AAAAAAAAAAAAAAAAAAAATTTTA62500.11839267079279331No Hit
TTTTTTTTTATTTTTTTTTTTTTTT61000.11555124669376628No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT57340.10861817189214029No Hit
TAATTTTTTTTTTTTTTTTTTTTTT56830.10765208769847111No Hit
TATTTTTTTTTTTTTTTTTTTTTTG55690.10549260538321055No Hit
TTTTTTATTTTTTTTTTTTTTTTTA53230.10083266986080622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)