FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299413
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2372174.476288222865438No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1408672.6581623285446896No Hit
AAAAAAAAAAAAAAAAAAAAATTTT814361.5366984984940786No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA596791.126143593639522No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG581331.0969705512667158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA451020.8510753926897187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG435550.8218834803024411No Hit
AAAAAAAAAAAAAAAAAAAAAATTT388890.7338359927788228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC350530.6614506172664784No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT349390.6592994356167372No Hit
AAAAAAAAAAAAAAAAAAAAATTTA287980.5434186767477832No Hit
TATTTTTTTTTTTTTTTTTTTTTTT273970.516981786473332No Hit
AAAAAAAAAAAAAAAAAAAATTTTT267560.5048861071971557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT244710.4617681241299744No Hit
AAAAAAAAAAAAAAAAAAAAAAATT189280.357171633914926No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA183990.34718939625954803No Hit
TTTATTTTTTTTTTTTTTTTTTTTT181360.3422265824535661No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT176300.33267835513103056No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC171520.32365848821369464No Hit
AAAAAAAAAAAAAAAAAAAAAATTA168660.31826166407487017No Hit
TTATTTTTTTTTTTTTTTTTTTTTT164400.3102230379100478No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG160040.30199571160051125No Hit
TATTTTTTTTTTTTTTTTTTTTTTA156240.29482510610137386No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT156210.29476849605795963No Hit
TTTTTATTTTTTTTTTTTTTTTTTT118030.2227227808061006No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT115220.21742030673963325No Hit
TTTTATTTTTTTTTTTTTTTTTTTT114090.21528799510436344No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC109840.20726823895401247No Hit
AAAAAAAAAAAAAAAAAAAAAATAA106500.20096565412056014No Hit
TTTTTTTTTTTTTTTTTTTTTTACC102090.19264397773866654No Hit
AAAAAAAAAAAAAAAAAAAATTTTA100140.18896432491674078No Hit
AAAAAAAAAAAAAAAAAAAAATTAA98840.18651122303545695No Hit
AAAAAAAAAAAAAAAAAAAAAAATA97370.18373733090815908No Hit
TTTATTTTTTTTTTTTTTTTTTTTA97250.1835108907345021No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA89730.1693206398519987No Hit
TTATTTTTTTTTTTTTTTTTTTTTA87600.1653013267695875No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT87510.1651314966393448No Hit
TTTTTTTTTTTTTTTTTTTTTTACA86810.16381059562634578No Hit
TTTTTTATTTTTTTTTTTTTTTTTT80690.15226214676984035No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC77410.14607278202321655No Hit
TTTTTTTTTTTTTTTTTTTTTAATT76540.1444310907642035No Hit
TATTTTTTTTTTTTTTTTTTTTTAA74980.1414873685066629No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC73490.1386757363504222No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA73470.13863799632147938No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA72450.13671325484539515No Hit
AAAAAAAAAAAAAAAAAAATTTTTT71590.13509043360085352No Hit
TTTTTTTATTTTTTTTTTTTTTTTT70080.13224106141567002No Hit
AAAAAAAAAAAAAAAAAAAAATAAA69310.13078807030137113No Hit
AATTTTTTTTTTTTTTTTTTTTTTT64940.12254187397736314No Hit
TTTTTTTTATTTTTTTTTTTTTTTT61610.11625815915838225No Hit
TTTTTATTTTTTTTTTTTTTTTTTA61210.11550335857952569No Hit
TTTTATTTTTTTTTTTTTTTTTTTA61170.11542787852164005No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA58300.11001218436834419No Hit
TATTTTTTTTTTTTTTTTTTTTTTG58300.11001218436834419No Hit
TAATTTTTTTTTTTTTTTTTTTTTT57310.1081440529356742No Hit
TAAAAAAAAAAAAAAAAAAAATTTT56550.10670993183584672No Hit
TATATTTTTTTTTTTTTTTTTTTTT53630.1011998876101938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)