FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578383
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2437825.324630988713701No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1492623.26014665002906No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA626071.3674478522220617No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG574271.2543074705632973No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC397300.8677736222592125No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT368510.8048911591712621No Hit
AAAAAAAAAAAAAAAAAAAAATTTT260970.5700047374804598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG246300.5379628571921572No Hit
TATTTTTTTTTTTTTTTTTTTTTTT219300.4789900713854651No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA190430.4159328741173467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA188600.4119358297460042No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT186260.4068248549760909No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC182240.39804446242265007No Hit
TTTATTTTTTTTTTTTTTTTTTTTT167890.36670151885501934No Hit
TTATTTTTTTTTTTTTTTTTTTTTT157730.34451027797368633No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG153160.3345285879315907No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT138370.30222460637303605No Hit
TATTTTTTTTTTTTTTTTTTTTTTA132540.2894908529932948No Hit
AAAAAAAAAAAAAAAAAAAAAATTT131410.28702273269842216No Hit
AAAAAAAAAAAAAAAAAAAATTTTT124030.2709035045779263No Hit
TTTTATTTTTTTTTTTTTTTTTTTT113340.24755464975298047No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC111470.24347023829155404No Hit
TTTTTATTTTTTTTTTTTTTTTTTT110570.2415044787646643No Hit
TTTTTTTTTTTTTTTTTTTTTTACC109910.24006292177827848No Hit
AAAAAAAAAAAAAAAAAAAAATTTA108620.23724533312306986No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT108000.23589114322676805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT98750.2156875036448458No Hit
TTTTTTTTTTTTTTTTTTTTTTACA97020.2119088769987133No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT96460.21068573773753746No Hit
TTTATTTTTTTTTTTTTTTTTTTTA91760.20042010465266885No Hit
TTATTTTTTTTTTTTTTTTTTTTTA89980.1965322691439314No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC88340.19295021845048788No Hit
TTTTTTATTTTTTTTTTTTTTTTTT87960.19212023109468998No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA84730.18506533857040794No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA83460.18229143346024132No Hit
TTTTTTTTTTTTTTTTTTTTTAATT79120.17281210418612858No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA77390.1690334775399961No Hit
TTTTTTTATTTTTTTTTTTTTTTTT74170.16200042678823506No Hit
AAAAAAAAAAAAAAAAAAAAAAATT70790.15461790767613806No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC68740.15014034430933368No Hit
TTTTTTTTATTTTTTTTTTTTTTTT64510.1409012745329519No Hit
TTTTATTTTTTTTTTTTTTTTTTTA64170.140158654267238No Hit
AAAAAAAAAAAAAAAAAAAAAATTA63390.13845499601060024No Hit
TTTTTATTTTTTTTTTTTTTTTTTA62090.13561556558287063No Hit
TATTTTTTTTTTTTTTTTTTTTTAA61600.13454531872934178No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60990.13321297060556098No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT57240.12502230591018706No Hit
TTTTTTTTTATTTTTTTTTTTTTTT55690.12163683116943252No Hit
AAAAAAAAAAAAAAAAAAAATTTTA51310.11207013480523582No Hit
AATTTTTTTTTTTTTTTTTTTTTTT49680.10850992588431331No Hit
TATTTTTTTTTTTTTTTTTTTTTTG47290.10328974225179502No Hit
TTTTTTATTTTTTTTTTTTTTTTTA46890.10241607135095512No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT46480.10152055867759424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)