FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4365506
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1913464.383134509493287No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1192842.7324209381455438No Hit
AAAAAAAAAAAAAAAAAAAAATTTT572811.3121273914180853No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA518581.1879035328321619No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG488291.118518678018081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA385960.8841128611437025No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC323410.7408305016646409No Hit
AAAAAAAAAAAAAAAAAAAAAATTT308760.7072719634333339No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT285290.6535095817071377No Hit
AAAAAAAAAAAAAAAAAAAAATTTA220280.5045921366274608No Hit
AAAAAAAAAAAAAAAAAAAATTTTT212610.4870225811165991No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT199960.4580454132922965No Hit
TATTTTTTTTTTTTTTTTTTTTTTT197230.4517918426867355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG192850.44175864149539595No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA158700.3635317417957964No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC156730.3590190919448971No Hit
AAAAAAAAAAAAAAAAAAAAAAATT147070.33689107287906606No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT144500.33100401190606543No Hit
TTTATTTTTTTTTTTTTTTTTTTTT144280.33050006116129493No Hit
AAAAAAAAAAAAAAAAAAAAAATTA138920.31822198847052324No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG134780.30873855172802417No Hit
TTATTTTTTTTTTTTTTTTTTTTTT129160.2958649008843419No Hit
TATTTTTTTTTTTTTTTTTTTTTTA123060.2818917211429786No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT116160.26608599323881355No Hit
TTTTTTTTTTTTTTTTTTTTTTACC95700.21921857397515893No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC95220.21811904507747787No Hit
TTTTTATTTTTTTTTTTTTTTTTTT92250.21131571002307634No Hit
TTTTATTTTTTTTTTTTTTTTTTTT92170.2111324552067962No Hit
AAAAAAAAAAAAAAAAAAAAAATAA90580.2074902657332277No Hit
AAAAAAAAAAAAAAAAAAAATTTTA88060.20171773902040221No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT84330.1931734832113391No Hit
AAAAAAAAAAAAAAAAAAAAAAATA82760.18957710744184064No Hit
TTTATTTTTTTTTTTTTTTTTTTTA81410.1864846824171127No Hit
TTTTTTTTTTTTTTTTTTTTTTACA77890.17842147050078502No Hit
TTATTTTTTTTTTTTTTTTTTTTTA77080.17656601548594825No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT76330.17484800158332162No Hit
AAAAAAAAAAAAAAAAAAAAATTAA75710.17342777675715026No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC75100.17203045878301393No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA71440.16364655093819594No Hit
TTTTTTATTTTTTTTTTTTTTTTTT68600.15714100496024974No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA65760.15063545898230354No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA64880.1486196560032216No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC64260.14719943117705028No Hit
TTTTTTTTTTTTTTTTTTTTTAATT60530.13865517536798713No Hit
TTTTTTTATTTTTTTTTTTTTTTTT56780.13006510585485395No Hit
TATTTTTTTTTTTTTTTTTTTTTAA56690.12985894418653873No Hit
AAAAAAAAAAAAAAAAAAAAATAAA54680.12525466692749934No Hit
TTTTTTTTATTTTTTTTTTTTTTTT52420.12007771836758442No Hit
TTTTATTTTTTTTTTTTTTTTTTTA51870.11881784150565822No Hit
TTTTTATTTTTTTTTTTTTTTTTTA50990.1168020385265763No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA49870.11423647109865387No Hit
TTTTTTTTTATTTTTTTTTTTTTTT45600.10445524527969954No Hit
TATTTTTTTTTTTTTTTTTTTTTTG45410.1040200150910341No Hit
AATTTTTTTTTTTTTTTTTTTTTTT45320.10381385342271893No Hit
TAAAAAAAAAAAAAAAAAAAATTTT45080.1032640889738784No Hit
AAAAAAAAAAAAAAAAAAATTTTTT44610.10218746692823237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)