FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_RELA_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3930871
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2481436.3126721787613995No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1477193.757920318423067No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA626251.5931583610858766No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG580821.4775860108357666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC381380.9702175421172559No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT354110.9008436043818279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG246330.6266550085210123No Hit
TATTTTTTTTTTTTTTTTTTTTTTT228700.5818048976931576No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA194450.4946740811387604No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC186120.4734828489665522No Hit
AAAAAAAAAAAAAAAAAAAAATTTT184290.46882739219882824No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT179550.456768995980789No Hit
TTTATTTTTTTTTTTTTTTTTTTTT170140.4328302811259896No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG160110.40731430769414717No Hit
TTATTTTTTTTTTTTTTTTTTTTTT155180.3947725580412077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA153550.39062589436285244No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT139820.3556972487776882No Hit
TATTTTTTTTTTTTTTTTTTTTTTA136300.3467424904047983No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC114530.29136036262701065No Hit
TTTTTTTTTTTTTTTTTTTTTTACC110970.28230384563624705No Hit
TTTTATTTTTTTTTTTTTTTTTTTT110940.28222752667284173No Hit
TTTTTATTTTTTTTTTTTTTTTTTT110580.28131169911197795No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT101290.25767826011079986No Hit
TTTTTTTTTTTTTTTTTTTTTTACA95430.2427706225922957No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT93830.23870027787734577No Hit
AAAAAAAAAAAAAAAAAAAAAATTT93830.23870027787734577No Hit
AAAAAAAAAAAAAAAAAAAATTTTT92370.23498608832495396No Hit
TTTATTTTTTTTTTTTTTTTTTTTA91730.23335795043897398No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC88230.22445407137502096No Hit
TTATTTTTTTTTTTTTTTTTTTTTA87170.2217574680013666No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA83820.21323518375444017No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA82870.21081841657993863No Hit
TTTTTTATTTTTTTTTTTTTTTTTT81830.20817269251522116No Hit
TTTTTTTTTTTTTTTTTTTTTAATT78080.19863282208955726No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA77380.19685204627676667No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC72950.185582279347249No Hit
TTTTTTTATTTTTTTTTTTTTTTTT67640.17207382282450887No Hit
TATTTTTTTTTTTTTTTTTTTTTAA62700.15950663351710093No Hit
AAAAAAAAAAAAAAAAAAAAATTTA61710.15698810772472563No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60990.15515645260299815No Hit
TTTTATTTTTTTTTTTTTTTTTTTA60080.15284144404637037No Hit
TTTTTTTTATTTTTTTTTTTTTTTT59720.15192561648550665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT58420.14861846140460983No Hit
TTTTTATTTTTTTTTTTTTTTTTTA56930.1448279528888127No Hit
TTTTTTTTTATTTTTTTTTTTTTTT54520.13869699616191933No Hit
AATTTTTTTTTTTTTTTTTTTTTTT51050.12986943606137163No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT49840.12679123787069074No Hit
AAAAAAAAAAAAAAAAAAAAAAATT47930.12193226386721925No Hit
TATTTTTTTTTTTTTTTTTTTTTTG47830.12167786732253487No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT46290.11776016053439556No Hit
TAATTTTTTTTTTTTTTTTTTTTTT46240.11763296226205337No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC42760.10877996250703724No Hit
TTTTTTATTTTTTTTTTTTTTTTTA42480.108067652181921No Hit
TTTTTTTTTTTTTTTTTTTTTAACA42330.10768605736489445No Hit
TATATTTTTTTTTTTTTTTTTTTTT42310.10763517805595757No Hit
TTTTTTTTTTTTTTTTTTTTAAATT41710.10610879878785134No Hit
TTTTTTTTTTTTTTTTTTTTTTATT40080.10196213510949609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)