FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335109
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2255743.5606964300061765No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1436302.2672064521699626No Hit
AAAAAAAAAAAAAAAAAAAAATTTT621150.9804882599494342No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA557700.88033213003912No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG509430.8041377030766163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA439540.6938160022187463No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT415300.6555530457329148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG346120.5463520832869648No Hit
AAAAAAAAAAAAAAAAAAAAAATTT321280.5071420239178205No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC314900.49707116325859585No Hit
AAAAAAAAAAAAAAAAAAAATTTTT306700.48412742385332286No Hit
AAAAAAAAAAAAAAAAAAAAATTTA274320.43301543825054944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT260180.410695380300481No Hit
TATTTTTTTTTTTTTTTTTTTTTTT255410.40316591237814536No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT201970.31881061557109747No Hit
TTTATTTTTTTTTTTTTTTTTTTTT193390.3052670443397264No Hit
AAAAAAAAAAAAAAAAAAAAAAATT178660.2820156685544006No Hit
TTATTTTTTTTTTTTTTTTTTTTTT171670.27098191996380805No Hit
AAAAAAAAAAAAAAAAAAAAAATTA165290.26091105930458336No Hit
TATTTTTTTTTTTTTTTTTTTTTTA158170.24967210508927312No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA154270.2435159363477408No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT152000.2399327304392079No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC149910.2366336553956688No Hit
AAAAAAAAAAAAAAAAAAAATTTTA141600.22351628046178842No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG128210.20238010111586083No Hit
TTTTTATTTTTTTTTTTTTTTTTTT124090.19587666131711387No Hit
TTTTATTTTTTTTTTTTTTTTTTTT117560.18556902493706107No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT113930.17983905249301946No Hit
AAAAAAAAAAAAAAAAAAAAAATAA113850.17971277210857775No Hit
TTTATTTTTTTTTTTTTTTTTTTTA109620.1730356967812235No Hit
TTATTTTTTTTTTTTTTTTTTTTTA104070.16427499511058136No Hit
AAAAAAAAAAAAAAAAAAAAATTAA100620.15882915353153357No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT97770.1543304148357984No Hit
TTTTTTTTTTTTTTTTTTTTTAATT96800.15279926517444295No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA95120.1501473771011675No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC92420.1458854141262605No Hit
TTTTTTATTTTTTTTTTTTTTTTTT91850.14498566638711347No Hit
AAAAAAAAAAAAAAAAAAAAAAATA89890.1418917969682921No Hit
AAAAAAAAAAAAAAAAAAATTTTTT84350.13314688034570518No Hit
TTTTTTTTTTTTTTTTTTTTTTACC83310.1315052353479632No Hit
TTTTTTTATTTTTTTTTTTTTTTTT75970.11991901007543833No Hit
TTTTTTTTTTTTTTTTTTTTTTACA72530.11448895354444573No Hit
TTTTTATTTTTTTTTTTTTTTTTTA71380.11267367301809644No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC70250.11088996258785759No Hit
TTTTTTTTATTTTTTTTTTTTTTTT68810.10861691566790721No Hit
TATTTTTTTTTTTTTTTTTTTTTAA68660.10838013994707905No Hit
TTTTATTTTTTTTTTTTTTTTTTTA68130.10754353240015285No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA68010.10735411182349033No Hit
AAAAAAAAAAAAAAAAAAAAATAAA65250.10299743856025208No Hit
AATTTTTTTTTTTTTTTTTTTTTTT65140.10282380303164476No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA64170.10129265337028928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)