FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7579187
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT3248914.286620715388075No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1990562.626350293243853No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA789281.041378184757811No Hit
AAAAAAAAAAAAAAAAAAAAATTTT748700.9878368220760353No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG717940.9472519941782674No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG713610.941538980368211No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT559180.7377836171610491No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA542980.7164092929756186No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC427700.5643085465499136No Hit
AAAAAAAAAAAAAAAAAAAAAATTT368960.4868068303368158No Hit
TATTTTTTTTTTTTTTTTTTTTTTT339550.44800319612116707No Hit
AAAAAAAAAAAAAAAAAAAAATTTA298980.3944750274666663No Hit
AAAAAAAAAAAAAAAAAAAATTTTT294920.3891182523930337No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT284310.3751193894543043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT269150.35511724410546935No Hit
TTTATTTTTTTTTTTTTTTTTTTTT254540.33584077025675707No Hit
TTATTTTTTTTTTTTTTTTTTTTTT247200.3261563542369386No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA233870.30856871587942086No Hit
AAAAAAAAAAAAAAAAAAAAAAATT211450.2789877067289671No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC203910.26903941016364946No Hit
TATTTTTTTTTTTTTTTTTTTTTTA198350.26170353099877336No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT195350.2577453228162862No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG184720.24372007182300687No Hit
AAAAAAAAAAAAAAAAAAAAAATTA173630.2290878955750795No Hit
TTTTTATTTTTTTTTTTTTTTTTTT163840.21617094287289654No Hit
TTTTATTTTTTTTTTTTTTTTTTTT161440.21300437632690686No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT158040.20851840705342142No Hit
TTATTTTTTTTTTTTTTTTTTTTTA140580.1854816354313464No Hit
TTTATTTTTTTTTTTTTTTTTTTTA140340.18516497877674742No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT129640.17104736959254338No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC129370.17069113085611953No Hit
AAAAAAAAAAAAAAAAAAAATTTTA124110.1637510725094921No Hit
TTTTTTTTTTTTTTTTTTTTTAATT120670.1592123271269069No Hit
AAAAAAAAAAAAAAAAAAAAAATAA118970.1569693424901642No Hit
TTTTTTTTTTTTTTTTTTTTTTACC117500.15502982048074548No Hit
AAAAAAAAAAAAAAAAAAAAAAATA117350.15483191007162114No Hit
TTTTTTATTTTTTTTTTTTTTTTTT116250.15338056707137587No Hit
AAAAAAAAAAAAAAAAAAAAATTAA110930.1463613445610987No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA110820.14621621026107418No Hit
TTTTTTTTTTTTTTTTTTTTTTACA107410.1417170469603138No Hit
TTTTTTTATTTTTTTTTTTTTTTTT107360.14165107682393902No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA92360.12186003591150343No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC91520.12075173762040704No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC90850.11986773779298493No Hit
TATTTTTTTTTTTTTTTTTTTTTAA90320.1191684543474122No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA89530.11812612619269058No Hit
TTTTATTTTTTTTTTTTTTTTTTTA89090.11754558899259247No Hit
TTTTTATTTTTTTTTTTTTTTTTTA88710.11704421595614412No Hit
AAAAAAAAAAAAAAAAAAATTTTTT88700.11703102192886915No Hit
TTTTTTTTATTTTTTTTTTTTTTTT87240.11510469394672541No Hit
TTTTTTTTTATTTTTTTTTTTTTTT78970.10419323339033594No Hit
AAAAAAAAAAAAAAAAAAAAATAAA76760.10127735336257042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)