FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6596815
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2624663.9786775891092896No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1776942.6936332154228975No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA698121.0582682703698678No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG622510.9436523534463223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG592790.8986003093917292No Hit
AAAAAAAAAAAAAAAAAAAAATTTT527900.8002346586951431No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT508260.7704627157196313No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA416370.6311682228469345No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC396440.6009566737887906No Hit
TATTTTTTTTTTTTTTTTTTTTTTT272790.41351773545263887No Hit
AAAAAAAAAAAAAAAAAAAAATTTA271210.4111226402438146No Hit
AAAAAAAAAAAAAAAAAAAAAATTT247080.3745443824027201No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT242190.36713171431971336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT231470.35088144809275384No Hit
AAAAAAAAAAAAAAAAAAAATTTTT217580.32982583261771026No Hit
TTTATTTTTTTTTTTTTTTTTTTTT209970.3182899626562212No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA204800.3104528473210178No Hit
TTATTTTTTTTTTTTTTTTTTTTTT190660.2890182610850842No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC184540.27974105685849915No Hit
TATTTTTTTTTTTTTTTTTTTTTTA181030.27442030737560474No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT164210.2489231545829313No Hit
AAAAAAAAAAAAAAAAAAAAAAATT160680.24357208743916572No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG157900.23935793257806987No Hit
AAAAAAAAAAAAAAAAAAAAAATTA145760.22095511242925564No Hit
TTTTTATTTTTTTTTTTTTTTTTTT135120.2048261168457809No Hit
TTTTATTTTTTTTTTTTTTTTTTTT132420.2007332326281698No Hit
TTTATTTTTTTTTTTTTTTTTTTTA128090.1941694590495565No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT124370.18853037412751458No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT124220.1883029916709806No Hit
TTATTTTTTTTTTTTTTTTTTTTTA122530.1857411493273648No Hit
AAAAAAAAAAAAAAAAAAAATTTTA118220.17920769340962267No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC114580.1736898791310655No Hit
TTTTTTTTTTTTTTTTTTTTTTACC108340.16423076893925326No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA106800.1618963090521714No Hit
TTTTTTTTTTTTTTTTTTTTTAATT102150.15484745289961896No Hit
TTTTTTATTTTTTTTTTTTTTTTTT99750.1512093335950758No Hit
AAAAAAAAAAAAAAAAAAAAAATAA99690.15111838061246222No Hit
AAAAAAAAAAAAAAAAAAAAATTAA97240.14740446715574107No Hit
TTTTTTTTTTTTTTTTTTTTTTACA96560.14637366668612048No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA87380.13245786034624285No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC86390.1309571361331188No Hit
AAAAAAAAAAAAAAAAAAAAAAATA86080.1304872123896153No Hit
TTTTTTTATTTTTTTTTTTTTTTTT84900.1286984703982149No Hit
TTTTATTTTTTTTTTTTTTTTTTTA82320.12478749214583097No Hit
TTTTTATTTTTTTTTTTTTTTTTTA81080.122907797171817No Hit
TATTTTTTTTTTTTTTTTTTTTTAA80730.12237723810657113No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC80330.12177088488914727No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA76680.11623791178015451No Hit
TTTTTTTTATTTTTTTTTTTTTTTT71470.1083401611232087No Hit
AAAAAAAAAAAAAAAAAAATTTTTT69700.10565704813610811No Hit
AAAAAAAAAAAAAAAAAAAAATAAA69170.10485363012302149No Hit
AATTTTTTTTTTTTTTTTTTTTTTT66570.10091233420976638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)