FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210778
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1575803.742301303939557No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1064262.527466420694703No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA432631.027434835082733No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG374110.8884581424145372No Hit
AAAAAAAAAAAAAAAAAAAAATTTT355680.8446895086846185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA322790.766580427654937No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT278990.6625616453776475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC253170.6012428107109897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG207970.4938992271736957No Hit
AAAAAAAAAAAAAAAAAAAAATTTA198640.47174180163380736No Hit
AAAAAAAAAAAAAAAAAAAAAATTT187060.4442409454974829No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT185070.43951497799219047No Hit
AAAAAAAAAAAAAAAAAAAATTTTT156500.3716652837076664No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT143850.34162332946548124No Hit
TATTTTTTTTTTTTTTTTTTTTTTT143680.3412196035981949No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA130020.30877904273272067No Hit
AAAAAAAAAAAAAAAAAAAAAAATT124830.2964535294902747No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC118960.28251311277868363No Hit
TTTATTTTTTTTTTTTTTTTTTTTT117880.27994826609239437No Hit
AAAAAAAAAAAAAAAAAAAAAATTA117140.27819087114067753No Hit
TTATTTTTTTTTTTTTTTTTTTTTT111730.26534288912880233No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG101510.2410718399307681No Hit
TATTTTTTTTTTTTTTTTTTTTTTA93860.2229041759028854No Hit
AAAAAAAAAAAAAAAAAAAATTTTA89910.21352348663358647No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT83360.19796816645285026No Hit
AAAAAAAAAAAAAAAAAAAAAATAA80370.19086734090469742No Hit
TTTTATTTTTTTTTTTTTTTTTTTT76920.18267408065682872No Hit
TTTTTATTTTTTTTTTTTTTTTTTT76350.18132041157239825No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC72700.17265217971595748No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT71730.1703485674143828No Hit
TTATTTTTTTTTTTTTTTTTTTTTA70810.16816369801495115No Hit
TTTATTTTTTTTTTTTTTTTTTTTA70630.16773622356723628No Hit
TTTTTTTTTTTTTTTTTTTTTTACC70560.16756998350423602No Hit
AAAAAAAAAAAAAAAAAAAAATTAA69460.16495763965708948No Hit
AAAAAAAAAAAAAAAAAAAAAAATA68900.16362771915308763No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT66850.15875926016522363No Hit
TTTTTTTTTTTTTTTTTTTTTTACA64270.15263212641464355No Hit
TTTTTTTTTTTTTTTTTTTTTAATT62380.14814364471363725No Hit
TTTTTTATTTTTTTTTTTTTTTTTT58470.13885794976605273No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA56520.1342269765824748No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC55050.13073593525946986No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA54370.12912103179032472No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA52160.12387259551560306No Hit
AAAAAAAAAAAAAAAAAAAAATAAA51300.12183021759874305No Hit
TTTTTTTATTTTTTTTTTTTTTTTT50390.11966909677974No Hit
TTTTATTTTTTTTTTTTTTTTTTTA48150.1143494147637325No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC47990.11396943747687482No Hit
TTTTTATTTTTTTTTTTTTTTTTTA47990.11396943747687482No Hit
TTTTTTTTATTTTTTTTTTTTTTTT42790.10162017565400028No Hit
TATTTTTTTTTTTTTTTTTTTTTAA42550.10105020972371377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)