FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5671399
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2229133.9304764133153034No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1459762.5738975515565032No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA599721.0574463196823218No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG532750.9393625805555208No Hit
AAAAAAAAAAAAAAAAAAAAATTTT452870.7985154985568818No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT390680.6888600149627985No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC350930.6187714883047375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA332040.5854640098501269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG267380.4714533398196811No Hit
AAAAAAAAAAAAAAAAAAAAAATTT221630.39078541291134694No Hit
TATTTTTTTTTTTTTTTTTTTTTTT220130.38814056284877857No Hit
AAAAAAAAAAAAAAAAAAAATTTTT213690.3767853399134852No Hit
AAAAAAAAAAAAAAAAAAAAATTTA208290.3672638796882392No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT196470.3464224611952007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT190870.33654835429494556No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA181940.32080268025578873No Hit
TTTATTTTTTTTTTTTTTTTTTTTT175130.3087950609717285No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC163670.2885884064937064No Hit
TTATTTTTTTTTTTTTTTTTTTTTT161220.2842684847248448No Hit
TATTTTTTTTTTTTTTTTTTTTTTA142720.251648667286502No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG140120.24706426051138353No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT134920.23789544696114664No Hit
AAAAAAAAAAAAAAAAAAAAAAATT131970.23269390850476224No Hit
AAAAAAAAAAAAAAAAAAAAAATTA118750.20938396328665995No Hit
TTTTTATTTTTTTTTTTTTTTTTTT116100.20471139484278925No Hit
TTTTATTTTTTTTTTTTTTTTTTTT113460.20005645873266897No Hit
TTTATTTTTTTTTTTTTTTTTTTTA105950.18681457608607682No Hit
TTATTTTTTTTTTTTTTTTTTTTTA101040.17815710021460315No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT100670.17750470386583628No Hit
AAAAAAAAAAAAAAAAAAAATTTTA100430.17708152785582534No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC100090.17648202850830985No Hit
TTTTTTTTTTTTTTTTTTTTTTACC97060.1711394313819218No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT94820.16718978862181977No Hit
TTTTTTTTTTTTTTTTTTTTTTACA89550.15789754873532968No Hit
TTTTTTATTTTTTTTTTTTTTTTTT89180.15724515238656284No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA84730.14939876386761008No Hit
TTTTTTTTTTTTTTTTTTTTTAATT84320.14867583818384142No Hit
AAAAAAAAAAAAAAAAAAAAAATAA81800.1442324900787266No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA77480.1366153218985298No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC77010.13578660221225838No Hit
AAAAAAAAAAAAAAAAAAAAATTAA74690.1316959007821527No Hit
TTTTTTTATTTTTTTTTTTTTTTTT73860.1302324170808649No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA71860.12670595033077375No Hit
TTTTATTTTTTTTTTTTTTTTTTTA70110.12362029192444404No Hit
TTTTTATTTTTTTTTTTTTTTTTTA68970.12161020587689211No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC68130.12012908984185384No Hit
AAAAAAAAAAAAAAAAAAAAAAATA67980.11986460483559701No Hit
TTTTTTTTATTTTTTTTTTTTTTTT66040.11644393208800861No Hit
TATTTTTTTTTTTTTTTTTTTTTAA63480.111930054647892No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA57140.10075115505010315No Hit
TTTTTTTTTATTTTTTTTTTTTTTT56860.10025744970509042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)