FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_NGFR_NT_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5805290
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2430754.187129325149992No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1648652.8399098064007138No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA666191.1475567973348446No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG581021.0008457803141617No Hit
AAAAAAAAAAAAAAAAAAAAATTTT432620.7452168625512248No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT425180.7324009653264523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC383990.6614484375457557No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA309800.5336512043326No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG299090.5152025135695202No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT225990.3892828782024671No Hit
TATTTTTTTTTTTTTTTTTTTTTTT222100.3825820932287621No Hit
AAAAAAAAAAAAAAAAAAAAAATTT210300.36225580461957974No Hit
AAAAAAAAAAAAAAAAAAAATTTTT208910.3598614367240913No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA197470.3401552721741722No Hit
TTTATTTTTTTTTTTTTTTTTTTTT193460.33324777918071274No Hit
AAAAAAAAAAAAAAAAAAAAATTTA186730.3216549044061537No Hit
TTATTTTTTTTTTTTTTTTTTTTTT178600.30765043606779335No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC175770.3027755719352522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT173260.2984519291887227No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG144790.24941045150199218No Hit
TATTTTTTTTTTTTTTTTTTTTTTA144380.24870419910116462No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT140610.242210122147214No Hit
TTTTATTTTTTTTTTTTTTTTTTTT124830.2150280175495109No Hit
AAAAAAAAAAAAAAAAAAAAAAATT121690.20961915769927084No Hit
TTTATTTTTTTTTTTTTTTTTTTTA117970.20321120908688453No Hit
TTTTTATTTTTTTTTTTTTTTTTTT117900.2030906294086945No Hit
TTATTTTTTTTTTTTTTTTTTTTTA110690.19067092255511783No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT110510.19036086052548623No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC107910.18588218676414098No Hit
AAAAAAAAAAAAAAAAAAAAAATTA107830.18574438141763805No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT107150.18457303597236313No Hit
TTTTTTTTTTTTTTTTTTTTTTACC102850.1771659985978306No Hit
TTTTTTTTTTTTTTTTTTTTTAATT95180.1639539110018621No Hit
AAAAAAAAAAAAAAAAAAAATTTTA93860.16168012278456373No Hit
TTTTTTTTTTTTTTTTTTTTTTACA93520.1610944500619263No Hit
TTTTTTATTTTTTTTTTTTTTTTTT91650.15787325008742026No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA91420.15747705971622433No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC84270.1451607068725249No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA83550.1439204587539985No Hit
TTTTTTTATTTTTTTTTTTTTTTTT79300.1365995497210303No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA77580.1336367347712173No Hit
TTTTATTTTTTTTTTTTTTTTTTTA77520.13353338076134008No Hit
AAAAAAAAAAAAAAAAAAAAAATAA76240.1313284952172932No Hit
TTTTTATTTTTTTTTTTTTTTTTTA73490.12659143643125495No Hit
AAAAAAAAAAAAAAAAAAAAATTAA70530.1214926386106465No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC70190.12090696588800903No Hit
TTTTTTTTATTTTTTTTTTTTTTTT67540.11634216378509944No Hit
TATTTTTTTTTTTTTTTTTTTTTAA64910.1118118130188156No Hit
TTTTTTTTTATTTTTTTTTTTTTTT61810.10647185584182703No Hit
AAAAAAAAAAAAAAAAAAAAAAATA61730.1063340504953241No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA59540.10256162913480635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)