FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELB_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELB_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5314144
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2240484.2160694177651195No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1573022.9600628059759013No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA635041.194999608591713No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG565561.0642541865632547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG477670.8988653675925982No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT404610.7613832067779872No Hit
AAAAAAAAAAAAAAAAAAAAATTTT357990.6736550609091512No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC355610.6691764468557871No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA279020.5250516357855564No Hit
TATTTTTTTTTTTTTTTTTTTTTTT220010.4140083520506784No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT209770.3947390209975492No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA186900.3517029271318203No Hit
AAAAAAAAAAAAAAAAAAAATTTTT183480.3452672716433729No Hit
AAAAAAAAAAAAAAAAAAAAAATTT178720.33631004353664484No Hit
TTTATTTTTTTTTTTTTTTTTTTTT172810.32518877922766115No Hit
TTATTTTTTTTTTTTTTTTTTTTTT165010.3105109684645354No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC162920.306578067888262No Hit
AAAAAAAAAAAAAAAAAAAAATTTA156800.2950616317510403No Hit
TATTTTTTTTTTTTTTTTTTTTTTA147310.2772036286559039No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG140710.26478394262556676No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT133460.2511411056983025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT133300.25084002240059733No Hit
TTTTTATTTTTTTTTTTTTTTTTTT112620.21192500617220758No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT111650.21009968867987017No Hit
TTTTATTTTTTTTTTTTTTTTTTTT110800.2085001836608116No Hit
TTTATTTTTTTTTTTTTTTTTTTTA108520.2042097466685133No Hit
TTATTTTTTTTTTTTTTTTTTTTTA106400.20022039297392016No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT100200.18855341518784588No Hit
AAAAAAAAAAAAAAAAAAAAAAATT98620.18558021762300758No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC95630.17995372349714273No Hit
TTTTTTTTTTTTTTTTTTTTTTACC92720.17447777102013043No Hit
AAAAAAAAAAAAAAAAAAAAAATTA91570.1723137348178747No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA90270.1698674330240204No Hit
TTTTTTTTTTTTTTTTTTTTTAATT85410.16072202785622672No Hit
TTTTTTATTTTTTTTTTTTTTTTTT83960.15799346047077384No Hit
TTTTTTTTTTTTTTTTTTTTTTACA83080.15633750233339555No Hit
AAAAAAAAAAAAAAAAAAAATTTTA80840.15212233616552356No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA74990.14111397809317927No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC73190.1377267909939964No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA72910.1371998952230124No Hit
TTTTTATTTTTTTTTTTTTTTTTTA72760.13691762963141382No Hit
TTTTATTTTTTTTTTTTTTTTTTTA70410.13249546869636952No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC68990.1298233544292364No Hit
TTTTTTTATTTTTTTTTTTTTTTTT68730.12933409407046553No Hit
TATTTTTTTTTTTTTTTTTTTTTAA67480.1269818808071441No Hit
AAAAAAAAAAAAAAAAAAAAAATAA64550.12146829291791866No Hit
TTTTTTTTATTTTTTTTTTTTTTTT63500.11949243377672868No Hit
AAAAAAAAAAAAAAAAAAAAATTAA59210.11141963785700952No Hit
TTTTTTTTTATTTTTTTTTTTTTTT58420.10993303907459037No Hit
AAAAAAAAAAAAAAAAAAAAAAATA55660.10473935218917665No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA54050.10170970150601866No Hit
GTTTTTTTTTTTTTTTTTTTTTTTT53280.10026073813581265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)