FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELB_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELB_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6276011
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2348423.7418991139435547No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1561702.488364026130611No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA633771.0098293326764405No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG602930.960689839453755No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG555290.8847817507012018No Hit
AAAAAAAAAAAAAAAAAAAAATTTT524540.8357856606688548No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT429540.6844156264225796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA383270.6106904529007359No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC352770.5620927050637738No Hit
TATTTTTTTTTTTTTTTTTTTTTTT259000.41268251441879245No Hit
AAAAAAAAAAAAAAAAAAAAAATTT253310.4036162460518313No Hit
AAAAAAAAAAAAAAAAAAAATTTTT232020.36969342469285027No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT225780.3597508034960423No Hit
AAAAAAAAAAAAAAAAAAAAATTTA224640.357934363085087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT202340.3224022392567508No Hit
TTTATTTTTTTTTTTTTTTTTTTTT189050.3012263681500877No Hit
TTATTTTTTTTTTTTTTTTTTTTTT181880.2898019139864478No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA179750.28640803848176816No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC169090.2694227272705545No Hit
TATTTTTTTTTTTTTTTTTTTTTTA165710.2640371407889502No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT149270.23784215802043687No Hit
AAAAAAAAAAAAAAAAAAAAAAATT141910.22611496378830437No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG139590.22241834821513218No Hit
TTTTTATTTTTTTTTTTTTTTTTTT128290.20441328098373315No Hit
AAAAAAAAAAAAAAAAAAAAAATTA123840.19732278990588129No Hit
TTTTATTTTTTTTTTTTTTTTTTTT120590.19214434136587713No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT119560.19050317152089122No Hit
TTTATTTTTTTTTTTTTTTTTTTTA112710.179588595367344No Hit
TTATTTTTTTTTTTTTTTTTTTTTA111740.17804302764925045No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT103780.16535981214819412No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC101720.16207747245822227No Hit
AAAAAAAAAAAAAAAAAAAATTTTA101010.16094618062332905No Hit
TTTTTTTTTTTTTTTTTTTTTAATT97490.15533752251230917No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA97370.1551463182585244No Hit
TTTTTTTTTTTTTTTTTTTTTTACC96630.153967225360185No Hit
AAAAAAAAAAAAAAAAAAAAAATAA91510.1458091771987015No Hit
AAAAAAAAAAAAAAAAAAAAATTAA88050.14029612121457402No Hit
TTTTTTATTTTTTTTTTTTTTTTTT87870.14000931483389686No Hit
TTTTTTTTTTTTTTTTTTTTTTACA84460.13457592728884638No Hit
AAAAAAAAAAAAAAAAAAATTTTTT80700.12858486067025696No Hit
TTTTTATTTTTTTTTTTTTTTTTTA77680.12377288695000693No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA77380.12329487631554503No Hit
TTTTTTTATTTTTTTTTTTTTTTTT76610.12206798235375942No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC76580.12202018129031324No Hit
TATTTTTTTTTTTTTTTTTTTTTAA75540.12036307775751189No Hit
AAAAAAAAAAAAAAAAAAAAAAATA74440.11861037209781819No Hit
TTTTATTTTTTTTTTTTTTTTTTTA73840.11765435082889435No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC69900.11137647782962776No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA69020.1099743133018728No Hit
TTTTTTTTATTTTTTTTTTTTTTTT67860.1081260055152867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)