FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELB_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELB_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5581611
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2606874.670461628372167No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1671922.995407598272255No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA658621.1799819084490122No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG579211.0377111554352318No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT460310.8246902193649825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC384000.6879734184270455No Hit
AAAAAAAAAAAAAAAAAAAAATTTT366970.6574625139587836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG346710.6211647497469817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA293750.5262817491222516No Hit
TATTTTTTTTTTTTTTTTTTTTTTT278520.49899572005286646No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT230900.41367984977813754No Hit
AAAAAAAAAAAAAAAAAAAAAATTT193820.34724741656127595No Hit
TTTATTTTTTTTTTTTTTTTTTTTT191960.34391504531576994No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA187060.33513621784104985No Hit
TTATTTTTTTTTTTTTTTTTTTTTT184040.3297255935607121No Hit
AAAAAAAAAAAAAAAAAAAATTTTT183810.3293135261486334No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC175640.31467617503262046No Hit
TATTTTTTTTTTTTTTTTTTTTTTA170900.30618400314891164No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT170090.30473280921941714No Hit
AAAAAAAAAAAAAAAAAAAAATTTA162920.29188705554722466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT156020.27952503318486366No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG148350.2657834807907609No Hit
TTTTTATTTTTTTTTTTTTTTTTTT130710.23417970188176854No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT126790.22715663990199245No Hit
TTTTATTTTTTTTTTTTTTTTTTTT126450.2265474967710935No Hit
AAAAAAAAAAAAAAAAAAAAAAATT112060.20076640955451752No Hit
TTTATTTTTTTTTTTTTTTTTTTTA111040.19893898016182066No Hit
TTATTTTTTTTTTTTTTTTTTTTTA109360.19592909645620235No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT108260.19395833926799988No Hit
TTTTTTTTTTTTTTTTTTTTTAATT107150.19196966610535918No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA106460.1907334638691231No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC102590.18379998176153803No Hit
TTTTTTTTTTTTTTTTTTTTTTACC100520.18009137505282974No Hit
AAAAAAAAAAAAAAAAAAAAAATTA100210.17953597984524539No Hit
TTTTTTATTTTTTTTTTTTTTTTTT97440.1745732549258628No Hit
TTTTTTTTTTTTTTTTTTTTTTACA91690.16427156962389533No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC85660.15346823703765813No Hit
TTTTTTTATTTTTTTTTTTTTTTTT85520.15321741339552325No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA84520.15142581595170285No Hit
AAAAAAAAAAAAAAAAAAAATTTTA82120.1471259820865338No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA79210.14191243352501634No Hit
TATTTTTTTTTTTTTTTTTTTTTAA76300.13669888496349888No Hit
TTTTTATTTTTTTTTTTTTTTTTTA75830.1358568341649033No Hit
TTTTATTTTTTTTTTTTTTTTTTTA74830.13406523672108284No Hit
TTTTTTTTATTTTTTTTTTTTTTTT74310.13313360605029623No Hit
AAAAAAAAAAAAAAAAAAAAAATAA71970.12894126803175643No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC70540.1263792836870932No Hit
AATTTTTTTTTTTTTTTTTTTTTTT68460.12265276100394672No Hit
TTTTTTTTTATTTTTTTTTTTTTTT60740.10882162873765298No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60590.10855288912107992No Hit
AAAAAAAAAAAAAAAAAAAAATTAA60290.10801540988793379No Hit
AAAAAAAAAAAAAAAAAAAAAAATA58600.10498761020787727No Hit
TAATTTTTTTTTTTTTTTTTTTTTT58560.10491594631012445No Hit
TATTTTTTTTTTTTTTTTTTTTTTG57180.10244354183765224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)