FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELB_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELB_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5212209
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2086274.002659908687469No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1643793.1537300211867945No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA688131.320227181987522No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG643461.2345245557114077No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC419680.8051864382260956No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT400060.7675440489819192No Hit
AAAAAAAAAAAAAAAAAAAAATTTT342970.6580127542851792No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG315260.604849114837874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA281030.5391763837559085No Hit
TATTTTTTTTTTTTTTTTTTTTTTT236700.45412607207423955No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA197670.3792441937765734No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT193240.37074491832541634No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC183400.35186616653322994No Hit
AAAAAAAAAAAAAAAAAAAAATTTA181580.34837436488061013No Hit
TATTTTTTTTTTTTTTTTTTTTTTA178110.3417169188725932No Hit
AAAAAAAAAAAAAAAAAAAAAATTT172790.33151011404185826No Hit
AAAAAAAAAAAAAAAAAAAATTTTT171330.328708998430416No Hit
TTTATTTTTTTTTTTTTTTTTTTTT156630.30050598508233267No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG150280.28832305074489534No Hit
TTATTTTTTTTTTTTTTTTTTTTTT148640.2851765921128643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT147110.2822411764378596No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT139870.2683507127208445No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT111640.21418941565850488No Hit
TTATTTTTTTTTTTTTTTTTTTTTA110800.21257781489575725No Hit
TTTATTTTTTTTTTTTTTTTTTTTA108790.2087214844991826No Hit
AAAAAAAAAAAAAAAAAAAAAATTA107630.20649594058872162No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA106100.20356052491371698No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC104220.19995360892090092No Hit
TTTTTATTTTTTTTTTTTTTTTTTT104140.19980012313397258No Hit
TTTTATTTTTTTTTTTTTTTTTTTT103030.1976705078403418No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT100100.19204909089409117No Hit
TTTTTTTTTTTTTTTTTTTTTTACC100060.19197234800062699No Hit
TTTTTTTTTTTTTTTTTTTTTTACA95820.18383760129342472No Hit
AAAAAAAAAAAAAAAAAAAAAAATT92970.17836967013410246No Hit
AAAAAAAAAAAAAAAAAAAATTTTA91350.17526158294880348No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC83820.16081473325417303No Hit
TATTTTTTTTTTTTTTTTTTTTTAA83180.15958684695874628No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA82860.1589729038110329No Hit
TTTTTTATTTTTTTTTTTTTTTTTT78170.1499747995523587No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA77790.14924574206444907No Hit
TTTTTTTTTTTTTTTTTTTTTAATT77600.14888121332049425No Hit
AAAAAAAAAAAAAAAAAAAAAATAA77100.1479219271521921No Hit
TTTTTATTTTTTTTTTTTTTTTTTA73250.1405354236562655No Hit
TTTTATTTTTTTTTTTTTTTTTTTA72770.13961450893469543No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC69040.13245823411916138No Hit
AAAAAAAAAAAAAAAAAAAAATTAA66460.12750831749072225No Hit
TATTTTTTTTTTTTTTTTTTTTTTG66000.12662577421588428No Hit
TTTTTTTATTTTTTTTTTTTTTTTT65160.12501417345313667No Hit
AATTTTTTTTTTTTTTTTTTTTTTT59890.11490329723923196No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA58960.11311902496618997No Hit
TTTTTTTTATTTTTTTTTTTTTTTT57610.11052895231177413No Hit
TTTTTTATTTTTTTTTTTTTTTTTA55430.10634646461797675No Hit
AAAAAAAAAAAAAAAAAAAAAAATA55150.10580926436372755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)