FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELB_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELB_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4852795
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1925713.9682492254463666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1352522.7870948597663823No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA603921.2444786973280346No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG565961.1662557350969904No Hit
AAAAAAAAAAAAAAAAAAAAATTTT563451.1610834580896163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA503111.036742743099595No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC370960.7644254496635444No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT341660.7040478734420061No Hit
AAAAAAAAAAAAAAAAAAAAATTTA326490.6727875379034144No Hit
AAAAAAAAAAAAAAAAAAAAAATTT290460.5985416651640961No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT289600.5967694905719282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG233730.4816399621249198No Hit
TATTTTTTTTTTTTTTTTTTTTTTT225730.4651546170814963No Hit
AAAAAAAAAAAAAAAAAAAATTTTT205980.42445642150554475No Hit
AAAAAAAAAAAAAAAAAAAAAATTA195590.40304607963039857No Hit
AAAAAAAAAAAAAAAAAAAAAAATT182420.37590708035266274No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA179330.36953961582964046No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC177060.36486189917356904No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT163020.33593011862236094No Hit
TATTTTTTTTTTTTTTTTTTTTTTA156270.3220206087419724No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG149260.3075753251476726No Hit
TTTATTTTTTTTTTTTTTTTTTTTT146210.30129028734986746No Hit
TTATTTTTTTTTTTTTTTTTTTTTT131010.26996813176736295No Hit
AAAAAAAAAAAAAAAAAAAAAATAA129600.2670625897034596No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT127520.26277639999216945No Hit
AAAAAAAAAAAAAAAAAAAATTTTA125370.2583459635117494No Hit
AAAAAAAAAAAAAAAAAAAAATTAA111840.23046512370705954No Hit
AAAAAAAAAAAAAAAAAAAAAAATA107810.22216063114143497No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC105680.2177714080236235No Hit
TTTTTTTTTTTTTTTTTTTTTTACC98680.20334673111062798No Hit
TTTATTTTTTTTTTTTTTTTTTTTA93090.1918275962615359No Hit
TTTTTATTTTTTTTTTTTTTTTTTT92870.19137424927284172No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT91290.1881183936267656No Hit
TTTTATTTTTTTTTTTTTTTTTTTT90100.1856661985515564No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA89610.1846564711676467No Hit
TTTTTTTTTTTTTTTTTTTTTTACA88520.18241034290548025No Hit
TTATTTTTTTTTTTTTTTTTTTTTA86240.17771201956810456No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT85670.17653743873376065No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC81750.16845961966248316No Hit
AAAAAAAAAAAAAAAAAAAAATAAA76510.15766171865904083No Hit
TATTTTTTTTTTTTTTTTTTTTTAA76020.15665199127513113No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA73550.15156214099297416No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA73200.15084090714732437No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC68830.14183578741735434No Hit
TTTTTTTTTTTTTTTTTTTTTAATT67510.13911570548518945No Hit
TTTTTTATTTTTTTTTTTTTTTTTT65890.1357774231138962No Hit
TTTTTATTTTTTTTTTTTTTTTTTA61830.12741111050435883No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA60900.12549468914306086No Hit
TTTTATTTTTTTTTTTTTTTTTTTA60850.12539165573653946No Hit
TATTTTTTTTTTTTTTTTTTTTTTG60510.12469102857219395No Hit
AATTTTTTTTTTTTTTTTTTTTTTT57040.11754051015960905No Hit
TTTTTTTATTTTTTTTTTTTTTTTT56460.11634532264396084No Hit
AAAAAAAAAAAAAAAAAAATTTTTT49990.10301279984009215No Hit
TTTTTTTTATTTTTTTTTTTTTTTT49370.10173518559922683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)