FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751953
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2141323.72277033557124No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1448342.5179969307815973No Hit
AAAAAAAAAAAAAAAAAAAAATTTT760781.3226464124446078No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA608071.0571539788311899No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG574160.9982000896043484No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG570100.9911416174645378No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA569280.9897160147170884No Hit
AAAAAAAAAAAAAAAAAAAAAATTT419320.7290045659274337No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT371100.6451721702176634No Hit
AAAAAAAAAAAAAAAAAAAAATTTA352460.6127657858122276No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC352040.6120355990391437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT318990.5545768541571879No Hit
AAAAAAAAAAAAAAAAAAAATTTTT310520.5398514208999969No Hit
TATTTTTTTTTTTTTTTTTTTTTTT268430.4666762749973792No Hit
AAAAAAAAAAAAAAAAAAAAAATTA231550.402558922160873No Hit
AAAAAAAAAAAAAAAAAAAAAAATT214150.37230832727597046No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT190300.33084414980442295No Hit
TATTTTTTTTTTTTTTTTTTTTTTA176950.30763464165997184No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA172560.30000245134130965No Hit
TTTATTTTTTTTTTTTTTTTTTTTT171860.2987854733861699No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC164790.2864939960392583No Hit
TTATTTTTTTTTTTTTTTTTTTTTT156130.2714382401942436No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT153550.26695280715958564No Hit
AAAAAAAAAAAAAAAAAAAAAATAA152380.26491871543456635No Hit
AAAAAAAAAAAAAAAAAAAATTTTA149170.25933800224028253No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG145510.2529749460748375No Hit
AAAAAAAAAAAAAAAAAAAAAAATA126110.21924727131810712No Hit
AAAAAAAAAAAAAAAAAAAAATTAA124830.21702194020013724No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT113520.19735905352495056No Hit
TTTTTATTTTTTTTTTTTTTTTTTT112410.1954292741960861No Hit
TTTTATTTTTTTTTTTTTTTTTTTT107920.1876232298838325No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC103510.17995626876645202No Hit
TTTATTTTTTTTTTTTTTTTTTTTA103230.17946947758439613No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA101120.1758011583196177No Hit
TTATTTTTTTTTTTTTTTTTTTTTA98610.17143742308047372No Hit
TTTTTTTTTTTTTTTTTTTTTTACC92520.1608497148707578No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT86080.14965351768347201No Hit
TTTTTTTTTTTTTTTTTTTTTAATT83990.1460199692174119No Hit
TATTTTTTTTTTTTTTTTTTTTTAA81610.14188224416993672No Hit
TTTTTTTTTTTTTTTTTTTTTTACA81180.14113467199749372No Hit
TTTTTTATTTTTTTTTTTTTTTTTT79440.13810961250900347No Hit
AAAAAAAAAAAAAAAAAAAAATAAA79230.13774451912246152No Hit
AAAAAAAAAAAAAAAAAAATTTTTT76280.13261582631151542No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC75930.1320073373339455No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA71530.1243577616159242No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA70640.12281046107296077No Hit
AATTTTTTTTTTTTTTTTTTTTTTT70130.12192380570564466No Hit
TTTTTATTTTTTTTTTTTTTTTTTA69100.12013310957165331No Hit
TTTTATTTTTTTTTTTTTTTTTTTA66620.11582153053058673No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC66320.11529996854981256No Hit
TTTTTTTATTTTTTTTTTTTTTTTT66100.11491748976391149No Hit
TATTTTTTTTTTTTTTTTTTTTTTG64140.11150995148952016No Hit
TTTTTTTTATTTTTTTTTTTTTTTT59460.10337358458944292No Hit
TAATTTTTTTTTTTTTTTTTTTTTT58950.10248692922212681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)