FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676227
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1758554.78357294040874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1216433.3089088350637756No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA509941.3871287055995183No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG462551.2582193645822197No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG338620.9211074288938088No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT335850.9135725296615252No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC303000.8242146091631448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA229890.6253422326749681No Hit
TATTTTTTTTTTTTTTTTTTTTTTT192990.5249675822521297No Hit
AAAAAAAAAAAAAAAAAAAAATTTT190000.5168342433696287No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT153920.41869014073396443No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA147950.40245066477124514No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC144880.39409971147048317No Hit
TTTATTTTTTTTTTTTTTTTTTTTT127150.3458709160234121No Hit
TATTTTTTTTTTTTTTTTTTTTTTA126800.34491885294352065No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG125400.34111060062395493No Hit
TTATTTTTTTTTTTTTTTTTTTTTT117840.32054603809830023No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT111680.30378972789221126No Hit
AAAAAAAAAAAAAAAAAAAAATTTA107440.29225616372438373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT106410.2894543780892747No Hit
AAAAAAAAAAAAAAAAAAAAAATTT105990.288311902393405No Hit
AAAAAAAAAAAAAAAAAAAATTTTT96460.2623885848180757No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC90450.24604030164622587No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT87760.23872301683220323No Hit
TTTTTATTTTTTTTTTTTTTTTTTT82800.22523092290002766No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT81610.22199390842839684No Hit
TTTTTTTTTTTTTTTTTTTTTTACC81370.22134106517361415No Hit
TTTTATTTTTTTTTTTTTTTTTTTT80210.21818565610883114No Hit
TTTATTTTTTTTTTTTTTTTTTTTA79330.2157918975079613No Hit
TTATTTTTTTTTTTTTTTTTTTTTA76420.20787617304372116No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA74660.20308865584198146No Hit
TTTTTTTTTTTTTTTTTTTTTTACA73320.19944361433611146No Hit
AAAAAAAAAAAAAAAAAAAAAAATT70540.1918815133015453No Hit
TTTTTTTTTTTTTTTTTTTTTAATT69240.18834527900480574No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC69140.18807326098197963No Hit
AAAAAAAAAAAAAAAAAAAAAATTA67120.18257849692089198No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA66420.18067437076110915No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA63080.1715889687987167No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC60130.16356443712534616No Hit
TATTTTTTTTTTTTTTTTTTTTTAA58250.1584504982962151No Hit
TTTTTTATTTTTTTTTTTTTTTTTT57640.15679118835697578No Hit
AAAAAAAAAAAAAAAAAAAATTTTA55720.15156844231871427No Hit
TTTTTATTTTTTTTTTTTTTTTTTA53850.14648170529186583No Hit
TTTTATTTTTTTTTTTTTTTTTTTA52710.14338069983164803No Hit
AAAAAAAAAAAAAAAAAAAAAATAA49190.1338056654281686No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA49180.133778463625886No Hit
TTTTTTTATTTTTTTTTTTTTTTTT48430.13173832845469008No Hit
AATTTTTTTTTTTTTTTTTTTTTTT47800.13002461491088554No Hit
TATTTTTTTTTTTTTTTTTTTTTTG45430.1235777877699065No Hit
TTTTTTTTATTTTTTTTTTTTTTTT44650.12145604719186273No Hit
AAAAAAAAAAAAAAAAAAAAAAATA42660.11604288853762294No Hit
AAAAAAAAAAAAAAAAAAAAATTAA41860.11386674435501398No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT38770.10546138744968686No Hit
TAATTTTTTTTTTTTTTTTTTTTTT38680.10521657122914337No Hit
TTTTTTTTTATTTTTTTTTTTTTTT38340.10429170995153456No Hit
TTTTTTATTTTTTTTTTTTTTTTTA38010.10339405047620837No Hit
TTTTTTTTTTTTTTTTTTTTAAATT37940.10320363786023007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)